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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RFX3

regulatory factor X, 3 (influences HLA class II expression)

Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.

Gene Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: RFX3, BA32F11.1, DNA binding protein RFX3, Transcription factor RFX3, Regulatory factor X 3
Target Sequences: NM_002919 NP_002910.1 P48380

Filtered by: None
Target
RFX3 (25)
Reactivity
Human (25)
Mouse (9)
Rat (6)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Horse (4)
Monkey (5)
Orangutan (3)
Pig (4)
Rabbit (4)
Application
IHC (4)
IHC-P (2)
WB (21)
ELISA (14)
IF (1)
Peptide-ELISA (1)
Host
rabbit (23)
mouse (2)
Isotype
IgG (16)
IgG2a (2)
Clonality
monoclonal mc (2)
polyclonal pc (23)
Clone
OTI3F7 (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
Carrier-free (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (13)
Epitope
aa661-690 (8)
N-Terminus (4)
610-690 aa, C-terminal (1)
C-Terminus (1)
aa641-690 (1)
aa694-739 (1)
Publications
No (25)
Showing 1-25 of 25 results

IHC-plus™ RFX3 Antibody (aa641-690) LS-B7703
Rabbit Polyclonal (IgG) to Human RFX3
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-RFX3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Western blot analysis of lysates from COLO and RAW264.7 cells, using RFX3 Antibody. The lane on the right is blocked with the synthesized peptide.

RFX3 Antibody (610-690 aa, C-terminal) LS-C385837
Rabbit Polyclonal (IgG) to Human RFX3
Mouse, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of RFX3 antibody

RFX3 Antibody LS-C801816
Rabbit Polyclonal (IgG) to Human RFX3
Mouse, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunofluorescence Image
Western blot Image

RFX3 Antibody (C-Terminus) LS-C352772
Rabbit Polyclonal to Human RFX3
Mouse, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of RFX3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of RFX3 expression in HepG2 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.

RFX3 Antibody (aa694-739) LS-C313917
Rabbit Polyclonal to Human RFX3
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $345.00

RFX3 Antibody (aa661-690) LS-C324632
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
Unconjugated
200µl  $590.00

RFX3 Antibody (aa661-690, Biotin) LS-C223880
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

RFX3 Antibody (aa661-690, FITC) LS-C223882
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

RFX3 Antibody (aa661-690, APC) LS-C223879
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
APC Conjugated
200µl  $740.00

RFX3 Antibody (aa661-690, Azide-free, HRP) LS-C223881
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

RFX3 Antibody (aa661-690, PE) LS-C223883
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
PE Conjugated
200µl  $740.00

RFX3 Antibody (aa661-690, AP) LS-C223878
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
AP Conjugated
200µl  $740.00

RFX3 Antibody LS-C752445
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA, WB
Unconjugated
100µg  $355.00

RFX3 Antibody (clone OTI3F7) LS-C174579
Mouse Monoclonal [clone OTI3F7] (IgG2a) to Human RFX3
Human
WB
Unconjugated
100µl 1mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFX3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFX3.

RFX3 Antibody (clone OTI3F7, Carrier-free) LS-C787927
Mouse Monoclonal [clone OTI3F7] (IgG2a) to Human RFX3
Human
WB
Unconjugated, Carrier-free
100µg  $395.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFX3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFX3.

RFX3 Antibody LS-C396575
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA
Unconjugated
50µg  $285.00; 100µg  $385.00

RFX3 Antibody (HRP) LS-C396576
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

RFX3 Antibody (Biotin) LS-C396578
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

RFX3 Antibody (FITC) LS-C396577
Rabbit Polyclonal (IgG) to Human RFX3
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

RFX3 Antibody LS-C750280
Rabbit Polyclonal (IgG) to Human RFX3
Human
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using RFX3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

RFX3 Antibody (aa661-690) LS-C162559
Rabbit Polyclonal to Human RFX3
Human
WB
Unconjugated
400µl  $325.00
RFX3 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The RFX3 antibody detected the RFX3 protein (arrow).

RFX3 Antibody (N-Terminus) LS-C201229
Rabbit Polyclonal to Human RFX3
Rabbit, Mouse, Dog, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Western blot of Human HeLa. RFX3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX3 Antibody (N-Terminus, HRP) LS-C426200
Rabbit Polyclonal to Human RFX3
Rabbit, Mouse, Dog, Pig, Horse, Rat, Chimpanzee, Orangutan, Gibbon, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Western blot of Human HeLa. RFX3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX3 Antibody (N-Terminus, Biotin) LS-C426196
Rabbit Polyclonal to Human RFX3
Rabbit, Mouse, Dog, Pig, Horse, Rat, Chimpanzee, Orangutan, Gibbon, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Western blot of Human HeLa. RFX3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX3 Antibody (N-Terminus, FITC) LS-C426198
Rabbit Polyclonal to Human RFX3
Rabbit, Mouse, Dog, Pig, Horse, Rat, Chimpanzee, Orangutan, Gibbon, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
Western blot of Human HeLa. RFX3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 25 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).