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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Rat IgG

Representing approximately 75% of serum antibodies, IgG is the most common type of antibody found in the circulation. IgG molecules are created and released by plasma B cells. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.

Rat IgG Target Details

Target Name: Rat IgG

Publications (1)

1
PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. Park CG, Rodriguez A, Steinman RM. Journal of immunological methods. 2012 384:184-90. (Flo; Mouse, Human, Hamster) [PubMed:22841832] [PMC:PMC3432670]


Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Goat Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
Unconjugated
2mg   2mg/ml   $225.00
Select
Goat Polyclonal (IgG) to Rat IgG
Rat
ELISA, Flo
Unconjugated, Fab'2, Azide-free, Low Endotoxin
0.5mg   0.5mg/ml   $405.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Goat Polyclonal (IgG) to Rat IgG
Rat
DB, ELISA, EM, IHC, WB
HRP Conjugated
2mg   2mg/ml   $255.00
Select
Goat Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC-Fr, IHC-P
HRP Conjugated
0.5mg   $315.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins)  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Donkey Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
HRP Conjugated
2mg   2mg/ml   $265.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (SHEEP) Antibody  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Sheep Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
HRP Conjugated
2mg   2mg/ml   $265.00
Rat IgG Antibody - ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
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Goat Polyclonal (IgG) to Rat IgG
Rat
FLISA, IF, WB
Atto 594 Conjugated
500µg   1mg/ml   $385.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (CHICKEN) Antibody  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Chicken Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
AP Conjugated
1000µg   1.2mg/ml   $305.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins)  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Donkey Polyclonal (IgG) to Rat IgG
Rat
FLISA, Flo, IF, WB
FITC Conjugated
2mg   2mg/ml   $245.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins)  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
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Donkey Polyclonal (IgG) to Rat IgG
Rat
FLISA, Flo, IF, WB
TR Conjugated
2mg   2mg/ml   $255.00
Rat IgG Antibody - Anti-Tubulin and Anti-TNFa Antibodies - Western Blot. DyLight dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight 680 conjugate. Anti-TNFa was detected using a DyLight 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.
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Goat Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
Unconjugated
1mg   1.1mg/ml   $265.00
Select
Rabbit Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
HRP Conjugated
1mg   $475.00
Select
Goat Polyclonal to Rat IgG
Rat
ELISA, IHC, WB
HRP Conjugated
1mg   $295.00
Rat IgG Antibody - Western Blot - Rat IgG (H&L) Antibody Biotin Conjugated. Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated Lane 1: Rat IgG Load: 100 ng per lane Primary antibody: Rat IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Goat Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
Biotin Conjugated
2mg   2mg/ml   $255.00
Select
Goat Polyclonal (IgG) to Rat IgG
Rat
ELISA, Flo, ICC, IF, IHC, WB
HRP Conjugated
250µl   $385.00
Select
Rabbit Monoclonal (IgG)
Rat
ELISA, IF, IHC, WB
Unconjugated
100µl   4mg/ml   $205.00; 1ml   4mg/ml   $355.00
Select
Goat Polyclonal to Rat IgG
Rat
ELISA, Flo, ID, IHC, IP, WB
Unconjugated
2mg   $195.00
Rat IgG Antibody - Western Blot of Unconjugated Anti-Rat Antibodies. Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
Select
Rabbit Polyclonal (IgG) to Rat IgG
Rat
FLISA, Flo, IF, WB
FITC Conjugated
2mg   2mg/ml   $245.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins)  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
Select
Donkey Polyclonal (IgG) to Rat IgG
Rat
FLISA, Flo, IF, WB
TRITC Conjugated
2mg   2mg/ml   $245.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins)  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  Lane 5: Rat Serum  All samples were reduced. Load: 50 ng per lane.
Select
Donkey Polyclonal (IgG) to Rat IgG
Rat
FLISA, Flo, IF, WB
TR Conjugated
1mg   1mg/ml   $305.00
Rat IgG Antibody - Dot Blot of Goat anti-Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed. Antigen: Rat IgG Load: 1 µg Primary antibody: Goat anti-Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed 1:1,000 in  Alkaline Phosphatase Substrate Neat for 30 seconds at RT.
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Goat Polyclonal (IgG) to Rat IgG
Rat
DB, ELISA, IHC, WB
AP Conjugated
1mg   1mg/ml   $345.00
Select
Rabbit Polyclonal (IgG F(ab')2) to Rat IgG
Rat
ELISA, IHC, WB
Unconjugated
1mg   1mg/ml   $255.00
Select
Rabbit Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
Unconjugated
1mg   $365.00
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody  Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule  Lane 2: Rat IgG F(c) Fragment  Lane 3: Rat IgG Fab Fragment  Lane 4: Rat IgM Whole Molecule  All samples were reduced. Load: 50 ng per lane.
Select
Rabbit Polyclonal (IgG) to Rat IgG
Rat
ELISA, IHC, WB
Unconjugated
2mg   2mg/ml   $235.00
Select
Mouse Monoclonal (IgG) to Rat IgG
Rat
ICC, IP, WB
Unconjugated
100µl   1mg/ml   $265.00; 200µl   1mg/ml   $305.00; 500µl   1mg/ml   $445.00; 1ml   1mg/ml   $545.00
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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).