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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAPGEF2

Rap guanine nucleotide exchange factor (GEF) 2

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

Gene Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: RAPGEF2, CNrasGEF, DKFZP586O1422, NRap GEP, NRAPGEP, PDZ-GEF1, Rap-GEP, KIAA0313, PDZGEF1, RA(Ras/Rap1A-associating)-GEF, RA-GEF
Target Sequences: AB002311 BAA20772.2 Q9Y4G8

Filtered By: None
Target
RAPGEF2 (15)
Reactivity
Human (15)
Mouse (10)
Rat (4)
Bat (4)
Bovine (4)
Chicken (4)
Dog (6)
Gibbon (3)
Guinea pig (6)
Horse (6)
Monkey (4)
Pig (6)
Rabbit (6)
Xenopus (4)
Zebrafish (4)
Application
IHC (6)
IHC-P (6)
WB (10)
ELISA (4)
IF (3)
Peptide-ELISA (1)
Host
rabbit (15)
Isotype
IgG (6)
Clonality
polyclonal pc (15)
Format
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (6)
Epitope
aa1274-1323 (4)
aa541-590 (4)
aa975-1025 (2)
aa1464-1498 (1)
Publications
No (15)
Showing 1-15 of 15 results

RAPGEF2 Antibody (aa975-1025) IHC-plus™ LS-B14427
Rabbit Polyclonal (IgG) to Human RAPGEF2
Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Human
IF IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human and Mouse PDZ-GEF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human and Mouse PDZ-GEF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

RAPGEF2 Antibody (aa1274-1323) IHC-plus™ LS-B12608
Rabbit Polyclonal to Mouse RAPGEF2
Mouse, Human
IHC IHC-P WB
Unconjugated
50µg  $485.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa1274-1323, HRP) LS-C444193
Rabbit Polyclonal to Mouse RAPGEF2
Mouse, Human
IHC IHC-P WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa1274-1323, Biotin) LS-C444190
Rabbit Polyclonal to Mouse RAPGEF2
Mouse, Human
IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa1274-1323, FITC) LS-C444191
Rabbit Polyclonal to Mouse RAPGEF2
Mouse, Human
IHC IHC-P WB
FITC Conjugated
100µl 1mg/ml  $385.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa1464-1498) LS-C356723
Rabbit Polyclonal to Human RAPGEF2
Human
Peptide-ELISA WB
Unconjugated
400µl  $305.00
All lanes : Anti-RAPGEF2 Antibody at 1:2000 dilution Lane 1: HeLa whole cell lysates Lane 2: HT-1080 whole cell lysates Lane 3: 293T/17 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 167 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RAPGEF2 Antibody at 1:2000 dilution Lane 1: HeLa whole cell lysates Lane 2: HT-1080 whole cell lysates Lane 3: 293T/17 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 167 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

RAPGEF2 Antibody (aa541-590) LS-C135117
Rabbit Polyclonal to Mouse RAPGEF2
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
50µg  $365.00
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa541-590, HRP) LS-C444195
Rabbit Polyclonal to Mouse RAPGEF2
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa541-590, Biotin) LS-C444194
Rabbit Polyclonal to Mouse RAPGEF2
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa541-590, FITC) LS-C444192
Rabbit Polyclonal to Mouse RAPGEF2
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon
WB
FITC Conjugated
100µl 1mg/ml  $385.00
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAPGEF2 antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAPGEF2 Antibody (aa975-1025) LS-C286803
Rabbit Polyclonal (IgG) to Human RAPGEF2
Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human PDZ-GEF1 by Immunohistochemistry. Sample: FFPE section of human lung adenocarcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Detection of Human PDZ-GEF1 by Immunohistochemistry. Sample: FFPE section of human lung adenocarcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

RAPGEF2 Antibody LS-C681179
Rabbit Polyclonal (IgG) to Human RAPGEF2
Human
ELISA IF
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

RAPGEF2 Antibody (HRP) LS-C681183
Rabbit Polyclonal (IgG) to Human RAPGEF2
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

RAPGEF2 Antibody (Biotin) LS-C681181
Rabbit Polyclonal (IgG) to Human RAPGEF2
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

RAPGEF2 Antibody (FITC) LS-C681182
Rabbit Polyclonal (IgG) to Human RAPGEF2
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00
Showing 1-15 of 15 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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