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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAP74 / GTF2F1

general transcription factor IIF, polypeptide 1, 74kDa

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Gene Name: general transcription factor IIF, polypeptide 1, 74kDa
Synonyms: GTF2F1, BTF4, TF2F1, TFIIF, TFIIF-alpha, RAP74
Target Sequences: NM_002096 NP_002087.2 P35269

Filtered By: None
Target
RAP74 / GTF2F1 (33)
Reactivity
Human (32)
Mouse (12)
Rat (7)
Bat (8)
Bovine (9)
Chimpanzee (6)
Dog (9)
Gibbon (6)
Guinea pig (9)
Hamster (8)
Horse (9)
Monkey (10)
Pig (9)
Rabbit (8)
Zebrafish (4)
Application
IHC (5)
IHC-P (3)
WB (26)
Flo (4)
ELISA (15)
IF (5)
IP (6)
Host
rabbit (25)
mouse (8)
Isotype
IgG (19)
IgG1 (8)
Clonality
monoclonal mc (8)
polyclonal pc (25)
Clone
1C5 (1)
1D5 (1)
2H3 (1)
4B10 (1)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (18)
Epitope
aa178-206 (8)
N-Terminus (4)
aa35-84 (4)
aa213-258 (1)
aa231-258 (1)
aa250-300 (1)
aa350-400 (1)
aa409-452 (1)
aa411-435 (1)
Publications
No (33)
Showing 1-25 of 33 results

RAP74 / GTF2F1 Antibody (clone 4B10) LS-C173333
Mouse Monoclonal [clone 4B10] (IgG1) to Human RAP74 / GTF2F1
Dog, Human, Monkey
Flo IHC IHC-P WB
Unconjugated
100µl 0.44mg/ml  $345.00
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-GTF2F1 mouse monoclonal antibody.
IHC of paraffin-embedded Human endometrium tissue using anti-GTF2F1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-GTF2F1 mouse monoclonal antibody.
IHC of paraffin-embedded Human endometrium tissue using anti-GTF2F1 mouse monoclonal antibody.

RAP74 / GTF2F1 Antibody (clone 2H3) LS-C173222
Mouse Monoclonal [clone 2H3] (IgG1) to Human RAP74 / GTF2F1
Mouse, Human, Monkey
Flo IF WB
Unconjugated
100µl 0.65mg/ml  $335.00
Anti-GTF2F1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.
Anti-GTF2F1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.

RAP74 / GTF2F1 Antibody LS-C346059
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded rat liver tissue.
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue.
Immunohistochemistry of paraffin-embedded rat liver tissue.
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue.

RAP74 / GTF2F1 Antibody (clone 1C5) LS-C337458
Mouse Monoclonal [clone 1C5] (IgG1) to Human RAP74 / GTF2F1
Human
Flo IF WB
Unconjugated
100µl 0.78mg/ml  $335.00
Anti-GTF2F1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1.
Western blot of extracts (35ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-GTF2F1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1.
Western blot of extracts (35ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

RAP74 / GTF2F1 Antibody (aa250-300) LS-C289469
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
IF IHC IHC-P IP
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human RAP74 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RAP74 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Detection of Human RAP74 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RAP74 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.

RAP74 / GTF2F1 Antibody (clone 1D5) LS-C337456
Mouse Monoclonal [clone 1D5] (IgG1) to Human RAP74 / GTF2F1
Human
Flo IF WB
Unconjugated
100µl 0.88mg/ml  $335.00
Anti-GTF2F1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.
Anti-GTF2F1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.

RAP74 / GTF2F1 Antibody (aa409-452) LS-C18143
Mouse Monoclonal (IgG1) to Human RAP74 / GTF2F1
Human
ELISA IP WB
Unconjugated
150µg  $515.00

RAP74 / GTF2F1 Antibody (aa213-258) LS-C18142
Mouse Monoclonal (IgG1) to Human RAP74 / GTF2F1
Human
ELISA IP WB
Unconjugated
150µg  $515.00

RAP74 / GTF2F1 Antibody (aa231-258) LS-C18141
Mouse Monoclonal (IgG1) to Human RAP74 / GTF2F1
Human
ELISA IP WB
Unconjugated
150µg  $515.00

RAP74 / GTF2F1 Antibody LS-C678151
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human colon cancer at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:100

RAP74 / GTF2F1 Antibody (Biotin) LS-C678150
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

RAP74 / GTF2F1 Antibody LS-C341743
Rabbit Polyclonal to Human RAP74 / GTF2F1
Mouse, Rat, Human
IHC WB
Unconjugated
100µl 1mg/ml  $385.00

RAP74 / GTF2F1 Antibody (aa411-435) LS-C18145
Mouse Monoclonal (IgG1)
ELISA IP WB
Unconjugated
50µg  $515.00

RAP74 / GTF2F1 Antibody (aa178-206) LS-C321823
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
Unconjugated
200µl  $530.00

RAP74 / GTF2F1 Antibody (aa178-206, Biotin) LS-C258147
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

RAP74 / GTF2F1 Antibody (aa178-206, FITC) LS-C258146
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
FITC Conjugated
200µl  $680.00

RAP74 / GTF2F1 Antibody (aa178-206, Azide-free, HRP) LS-C258148
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

RAP74 / GTF2F1 Antibody (aa178-206, PE) LS-C258149
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
PE Conjugated
200µl  $680.00

RAP74 / GTF2F1 Antibody (aa178-206, AP) LS-C258144
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
AP Conjugated
200µl  $680.00

RAP74 / GTF2F1 Antibody (aa178-206, APC) LS-C258145
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
ELISA WB
APC Conjugated
200µl  $680.00

RAP74 / GTF2F1 Antibody (aa35-84) LS-C110242
Rabbit Polyclonal to Human RAP74 / GTF2F1
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
50µg  $365.00
GTF2F1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2F1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAP74 / GTF2F1 Antibody (aa178-206) LS-C159798
Rabbit Polyclonal to Human RAP74 / GTF2F1
Human
WB
Unconjugated
400µl  $305.00
GTF2F1 Antibody western blot of T47D cell line lysates (35 ug/lane). The GTF2F1 antibody detected the GTF2F1 protein (arrow).
GTF2F1 Antibody western blot of T47D cell line lysates (35 ug/lane). The GTF2F1 antibody detected the GTF2F1 protein (arrow).

RAP74 / GTF2F1 Antibody (N-Terminus) LS-C30151
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Zebrafish, Pig, Horse, Bat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Hamster, Human, Monkey
WB
Unconjugated
50µg  $365.00
A: Marker, B: K562 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: K562 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAP74 / GTF2F1 Antibody (aa350-400) LS-C289468
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Human
IP
Unconjugated
100µl 1mg/ml  $395.00

RAP74 / GTF2F1 Antibody LS-C664293
Rabbit Polyclonal (IgG) to Human RAP74 / GTF2F1
Human
Unconjugated
100µg  $355.00
Showing 1-25 of 33 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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