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Unfortunately, the antibody (ID:18266) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



PTK2B / PYK2

protein tyrosine kinase 2 beta

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

Gene Name: protein tyrosine kinase 2 beta
Family/Subfamily: Protein Kinase , Focal Adhesion Kinase
Synonyms: PTK2B, CADTK, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, Focal adhesion kinase 2, FRNK, FAK2, Proline-rich tyrosine kinase 2, Protein-tyrosine kinase 2-beta, Protein kinase B, Protein tyrosine kinase 2 beta, PYK2, RAFTK, CAK-beta, PKB, PTK
Target Sequences: NM_004103 NP_004094.3 Q14289

Publications (2)

1
Microarray analysis sheds light on the dedifferentiating role of agouti signal protein in murine melanocytes via the Mc1r. Le Pape E, Passeron T, Giubellino A, Valencia JC, Wolber R, Hearing VJ. Proceedings of the National Academy of Sciences of the United States of America. 2009 106:1802-7. (WB) [PubMed:19174519] [PMC:PMC2644118]
2
Role of Pyk2 in cardiac arrhythmogenesis. Lang D, Glukhov AV, Efimova T, Efimov IR. American journal of physiology. Heart and circulatory physiology. 2011 301:H975-83. [PubMed:21666110] Related Antibodies: LS-B1892.

Filtered By: None
Target
ptk2b-pyk2 (98)
Reactivity
Human (86)
Mouse (67)
Rat (62)
Bat (4)
Bovine (7)
Chicken (2)
Chimpanzee (3)
Dog (8)
Gibbon (3)
Guinea pig (6)
Horse (8)
Monkey (8)
Orangutan (3)
Pig (9)
Rabbit (9)
Xenopus (2)
Application
IHC (55)
IHC-P (23)
WB (82)
ELISA (28)
ICC (2)
IF (5)
IP (20)
Peptide-ELISA (9)
Host
rabbit (85)
mouse (13)
Isotype
IgG (66)
IgG1 (3)
IgG1,k (7)
IgG2a (1)
IgG2b,k (1)
Clonality
monoclonal mc (13)
polyclonal pc (85)
Clone
1F9 (1)
5E2D5 (1)
Format
AP Conjugated (4)
APC (1)
APC Conjugated (5)
Azide-free (1)
Biotin Conjugated (8)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (8)
HRP Conjugated (7)
PE Conjugated (5)
Unconjugated (59)
Epitope
pTyr402 (11)
aa704-941 (10)
aa672-701 (8)
aa812-861 (4)
pTyr579 (4)
pTyr580 (4)
aa805-838 (3)
pTyr881 (3)
N-Terminus (2)
aa520-600 (2)
Arg875 (1)
Ile574 (1)
Internal (1)
aa275-325 (1)
aa340-420 (1)
aa369-418 (1)
aa5-416 (1)
aa545-594 (1)
aa546-595 (1)
aa726-863 (1)
aa769-802 (1)
aa820-900 (1)
aa847-896 (1)
aa851-885 (1)
aa959-1009 (1)
pTyr579/Tyr580 (1)
Publications
No (97)
Yes (1)
Showing 1-25 out of 98 results
PTK2B / PYK2 Antibody (N-Terminus) IHC-plus™ LS-B1892
Anti-PTK2B / PYK2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1892 dilution 1:50.
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-PYK2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Rabbit Polyclonal (IgG) to Mouse PTK2B / PYK2
Mouse, Human
IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (aa5-416) IHC-plus™ LS-B7507
Anti-PTK2B / PYK2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7507 dilution 1:50.

Mouse Monoclonal (IgG1) to Rat PTK2B / PYK2
Rat, Human
ELISA IHC IHC-P IP WB
Unconjugated
PTK2B / PYK2 Antibody (clone 5E2D5) IHC-plus™ LS-B4093
Anti-PTK2B / PYK2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4093 dilution 1:100.
Western blot using PYK2 mouse monoclonal antibody against Raji (1), PMA induced THP-1 (2), Jurkat (3) and Ramos (4) cell lysate.

Mouse Monoclonal [clone 5E2D5] (IgG2a) to Human PTK2B / PYK2
Human
ELISA IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (aa847-896) LS-C117575
IHC of paraffin-embedded human brain tissue, using PYK2 Antibody. The picture on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
PTK2B / PYK2 Antibody (aa369-418) IHC-plus™ LS-B8376
Anti-PTK2B antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8376 dilution 5 ug/ml.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA
Unconjugated
PTK2B / PYK2 Antibody (aa805-838) LS-C344310
Immunohistochemical of paraffin-embedded H. brain section using PTK2B Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of A549 cells stained with PTK2B Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Rabbit Polyclonal to Human PTK2B / PYK2
Human
IF IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (aa805-838) LS-C344308
Immunohistochemical of paraffin-embedded H.brain section using PTK2B Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of HeLa cells stained with XAF1 PTK2B Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Rabbit Polyclonal to Human PTK2B / PYK2
Human
IF IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (aa812-861) IHC-plus™ LS-B8064
Anti-PTK2B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8064 dilution 2.5-5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Raji Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Bat, Human, Monkey
IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody IHC-plus™ LS-B7894
Anti-PTK2B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7894 dilution 1:200.
Western blot Image

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr881) LS-C199509
IHC of paraffin-embedded human brain, using PYK2 (Phospho-Tyr881) Antibody. The sample on the right was incubated with synthetic peptide.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr579) LS-C199507
IHC of paraffin-embedded human brain, using PYK2 (Phospho-Tyr579) Antibody. The sample on the right was incubated with synthetic peptide.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr580) LS-C199508
IHC of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The sample on the right was incubated with synthetic peptide.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr402) LS-C335877
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed Hela cells.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IF IHC WB
Unconjugated
PTK2B / PYK2 Antibody (aa672-701) LS-C164562
Mouse Ptk2b Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Mouse Ptk2b Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The Ptk2b antibody detected the Ptk2b protein (arrow).

Rabbit Polyclonal to Mouse PTK2B / PYK2
Mouse, Human
IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (aa672-701) LS-C330038

Rabbit Polyclonal (IgG) to Mouse PTK2B / PYK2
Mouse, Human
ELISA IHC WB
Unconjugated
PTK2B / PYK2 Antibody LS-C330787
Western blot analysis of extracts of Jurkat cells.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Human
IF IHC WB
Unconjugated
PTK2B / PYK2 Antibody LS-C404958
Immunohistochemistry of Human brain using PTK2B Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of Human cervical cancer using PTK2B Polyclonal Antibody at dilution of 1:50.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
PTK2B / PYK2 Antibody (clone 1F9) LS-C197191
PTK2B monoclonal antibody (M01), clone 1F9 Western Blot analysis of PTK2B expression in K-562.
Western Blot analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.

Mouse Monoclonal [clone 1F9] (IgG2b,k) to Human PTK2B / PYK2
Human
ELISA IP WB
Unconjugated
PTK2B / PYK2 Antibody (aa546-595) LS-C117576
Western blot of extracts from COS cells, treated with UV 15', using PYK2 (Ab-580) Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
IHC Peptide-ELISA WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr579) LS-C357316
Immunohistochemistry of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody.
Western blot of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with synthesized peptide.

Rabbit Polyclonal to Human PTK2B / PYK2
Mouse, Human
IHC IHC-P WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr580) LS-C381209
Western blot of Phospho-PYK2 (Y580) antibody

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
PTK2B / PYK2 Antibody (aa520-600) LS-C385701
Western blot of PYK2 antibody

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr881) LS-C381213
Western blot of Phospho-PYK2 (Y881) antibody

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
PTK2B / PYK2 Antibody (phospho-Tyr579) LS-C381413
Western blot of Phospho-PYK2 (Y579) antibody

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
PTK2B / PYK2 Antibody (aa820-900) LS-C385695
Western blot of PYK2 antibody

Rabbit Polyclonal (IgG) to Human PTK2B / PYK2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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