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PRPF18

pre-mRNA processing factor 18

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18.

Gene Name: pre-mRNA processing factor 18
Synonyms: PRPF18, HPrp18, PRP18, PRP18 homolog, Pre-mRNA-splicing factor 18
Target Sequences: NM_003675 NP_003666.1 Q99633

☰ Filters
Antibodies (15)
Proteins (4)
Over-Expression Lysate (2)
Primary (15)
Recombinant (2)
PRPF18 (19)
Human (15)
Mouse (9)
Rat (12)
Bat (3)
Bovine (4)
Chimpanzee (6)
Dog (6)
Gibbon (6)
Guinea pig (6)
Hamster (3)
Horse (6)
Monkey (4)
Orangutan (3)
Pig (6)
Rabbit (6)
WB (15)
ELISA (2)
ICC (1)
IF (1)
IP (1)
Peptide-ELISA (2)
rabbit (15)
IgG (5)
polyclonal pc (15)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (9)
N-Terminus (4)
aa120-169 (4)
160-240 aa, Internal (1)
Internal (1)
aa182-231 (1)
aa223-268 (1)
No (19)
GST (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
PRPF18 Antibody - Western blot of PRPF18 antibody
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PRPF18 Rabbit anti-Human Polyclonal (160-240 aa, Internal) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
PRPF18 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Select
PRPF18 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
PRPF18 Rabbit anti-Human Polyclonal (aa223-268) Antibody
Mouse, Rat, Human
ICC, WB
Unconjugated
50 µl/$321; 100 µl/$403
PRPF18 Antibody - Western blot analysis of extracts from HuvEc cells, MCF-7 cells and 293 cells, using PRPF18 antibody.
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PRPF18 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
PRPF18 Antibody - Western blot analysis of lysates from 293, MCF-7, and HUVEC cells, using PRPF18 Antibody. The lane on the right is blocked with the synthesized peptide.
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PRPF18 Rabbit anti-Human Polyclonal (aa182-231) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
PRPF18 Antibody - Western blot analysis of PRPF18 expression in Jurkat (A); MCF7 (B) whole cell lysates.
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PRPF18 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Bovine, Rat, Human, Monkey
IP, WB
Unconjugated
100 µl/$299
PRPF18 Antibody - PRPF18 antibody Western blot of H226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF18 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
PRPF18 Antibody - PRPF18 antibody Western blot of H226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRPF18 Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Rabbit, Dog, Guinea pig, Rat, Pig, Horse, Gibbon, Chimpanzee, Human
WB
HRP Conjugated
100 µl/$469
PRPF18 Antibody - PRPF18 antibody Western blot of H226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRPF18 Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Rabbit, Dog, Guinea pig, Rat, Pig, Horse, Gibbon, Chimpanzee, Human
WB
Biotin Conjugated
100 µl/$469
PRPF18 Antibody - PRPF18 antibody Western blot of H226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF18 Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Rabbit, Dog, Guinea pig, Rat, Pig, Horse, Gibbon, Chimpanzee, Human
WB
FITC Conjugated
100 µl/$469
PRPF18 Antibody - Western blot of Human Placenta . PRPF18 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRPF18 Rabbit anti-Human Polyclonal (aa120-169) Antibody
Human
WB
Unconjugated
100 µl/$424
PRPF18 Antibody - Western blot of Human Placenta . PRPF18 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF18 Rabbit anti-Human Polyclonal (aa120-169) (HRP) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100 µl/$469
PRPF18 Antibody - Western blot of Human Placenta . PRPF18 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF18 Rabbit anti-Human Polyclonal (aa120-169) (Biotin) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
PRPF18 Antibody - Western blot of Human Placenta . PRPF18 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF18 Rabbit anti-Human Polyclonal (aa120-169) (FITC) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100 µl/$469
PRPF18 Antibody - Western blot analysis of extracts of HeLa cells, using PRPF18 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Select
PRPF18 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
PRPF18 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
39.7 kDa
100 µg/$494
PRPF18 Protein - 12.5% SDS-PAGE of human PRPF18 stained with Coomassie Blue
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Wheat Germ Extract
GST
63.36 kDa
10 µg/$479; 25 µg/$670
PRPF18 Protein - Purified recombinant protein PRPF18 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
39.7 kDa
20 µg/$1,107
Select
293T Cells
Myc-DDK (Flag)
39.7 kDa
20 µg/$150
Viewing 1-19 of 19 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).