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PRKAB1 / AMPK Beta 1

protein kinase, AMP-activated, beta 1 non-catalytic subunit

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Gene Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit
Family/Subfamily: Protein Kinase Non Catalytic , AMPK/SNF1
Synonyms: PRKAB1, AMPK beta 1, AMPK subunit beta-1, AMPKb, AMPK, AMPK beta -1 chain, HAMPKb
Target Sequences: NM_006253 NP_006244.2 Q9Y478

Publications (1)

1
Association of AMP-activated protein kinase subunits with glycogen particles as revealed in situ by immunoelectron microscopy. Bendayan M, Londono I, Kemp BE, Hardie GD, Ruderman N, Prentki M. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2009 57:963-71. (WB) [PubMed:19581628] [PMC:PMC2746729]

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Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
PRKAB1 / AMPK Beta 1 (7)
No (7)
GST (2)
His (1)
His-GST-S (1)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (3)
PRKAB1 / AMPK Beta 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
30.2 kDa
100 µg/$494
PRKAB1 / AMPK Beta 1 Protein
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E. coli
His
10 µg/$304; 50 µg/$479; 20 µg/$373; 100 µg/$739; 250 µg/$1,221; 500 µg/$1,801
PRKAB1 / AMPK Beta 1 Protein - Recombinant  Protein Kinase, AMP Activated Beta 1 By SDS-PAGE
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E. coli
His-GST-S
55.9 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
PRKAB1 / AMPK Beta 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
30.2 kDa
20 µg/$150
PRKAB1 / AMPK Beta 1 Protein - Purified recombinant protein PRKAB1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
30.2 kDa
20 µg/$1,107
PRKAB1 / AMPK Beta 1 Protein - 12.5% SDS-PAGE of human PRKAB1 stained with Coomassie Blue
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Wheat Germ Extract
GST
55.44 kDa
10 µg/$479; 25 µg/$670
PRKAB1 / AMPK Beta 1 Protein - 12.5% SDS-PAGE of human PRKAB1 stained with Coomassie Blue
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Wheat Germ Extract
GST
55.44 kDa
10 µg/$479; 25 µg/$670
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).