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PRKAA2 / AMPK Alpha 2

protein kinase, AMP-activated, alpha 2 catalytic subunit

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Gene Name: protein kinase, AMP-activated, alpha 2 catalytic subunit
Family/Subfamily: Protein Kinase , AMPK/SNF1
Synonyms: PRKAA2, ACACA kinase, Alpha2 AMPK, AMPK alpha 2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, AMPK-2, AMPKa2, AMPKalpha2, Ampk alpha2, AMPK2, PRKAA, AMPK, AMPK-alpha-2 chain, HMGCR kinase
Target Sequences: NM_006252 NP_006243.2 P54646

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Proteins (8)
Over-Expression Lysate (2)
Recombinant (6)
PRKAA2 / AMPK Alpha 2 (8)
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GST (1)
GST, N-terminus (1)
His, N-terminus (1)
His-T7 (1)
Myc-DDK (Flag) (2)
Human (6)
Rat (1)
293T Cells (1)
Baculovirus (2)
E. coli (2)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (3)
Low endotoxin level (2)
PRKAA2 / AMPK Alpha 2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
62.1 kDa
20 µg/$150
PRKAA2 / AMPK Alpha 2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
62.1 kDa
100 µg/$494
PRKAA2 / AMPK Alpha 2 Protein - Recombinant Rat AMPK alpha2 Protein (His Tag)-Elabscience
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E. coli
His, N-terminus
20 kDa
20 µg/$270; 100 µg/$390
PRKAA2 / AMPK Alpha 2 Protein - Recombinant  Protein Kinase, AMP Activated Alpha 2 By SDS-PAGE
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E. coli
His-T7
31.4 kDa
10 µg/$284; 50 µg/$462; 100 µg/$737; 200 µg/$922; 1 mg/$2,042; 500 µg/$1,577; 5 mg/$3,221; 2 mg/$2,231
PRKAA2 / AMPK Alpha 2 Protein - 12.5% SDS-PAGE of human PRKAA2 stained with Coomassie Blue
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Wheat Germ Extract
GST
88.7 kDa
2 µg/$439
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Baculovirus
None
Less than 1.0 EU/µg protein (determined by LAL method).
160 kDa
50 µg/$1,133
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Baculovirus
None
Less than 1.0 EU/µg protein (determined by LAL method).
158 kDa
50 µg/$1,133
PRKAA2 / AMPK Alpha 2 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).