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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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PRKAA2 / AMPK Alpha 2

protein kinase, AMP-activated, alpha 2 catalytic subunit

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Gene Name: protein kinase, AMP-activated, alpha 2 catalytic subunit
Family/Subfamily: Protein Kinase , AMPK/SNF1
Synonyms: PRKAA2, ACACA kinase, Alpha2 AMPK, AMPK alpha 2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, AMPK-2, AMPKa2, AMPKalpha2, Ampk alpha2, AMPK2, PRKAA, AMPK, AMPK-alpha-2 chain, HMGCR kinase
Target Sequences: NM_006252 NP_006243.2 P54646


Select
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, WB
Unconjugated
100µg   0.5mg/ml   $275.00
PRKAA2 / AMPK Alpha 2 Antibody - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
Select
Mouse Monoclonal [clone 8E11H5] (IgG1) to Human PRKAA2 / AMPK Alpha 2
Human, Monkey
ELISA, Flo, IHC, WB
Unconjugated
100µl   $365.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
APC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IHC, WB
Unconjugated
200µl   $590.00
PRKAA2 / AMPK Alpha 2 Antibody - Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Select
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
IHC, IHC-P, WB
Unconjugated
400µl   $325.00
PRKAA2 / AMPK Alpha 2 Antibody - IHC of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at 1:500 dilution.
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ICC, IHC, IHC-P, IP, WB
Unconjugated
100µl   0.96mg/ml   $355.00
PRKAA2 / AMPK Alpha 2 Antibody - Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:100
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
PRKAA2 / AMPK Alpha 2 Antibody - Immunofluorescence of monoclonal antibody to PRKAA2 on HeLa cell . [antibody concentration 10 ug/ml]
Select
Mouse Monoclonal [clone 1G8] (IgG2a,k) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IF, RNAi, WB
Unconjugated
100µg   $365.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg   $405.00
PRKAA2 / AMPK Alpha 2 Antibody - PRKAA2 (Thr172) antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.This data demonstrates the use of the PRKAA2 (Thr172) antibody for immunohistochemistry.
Select
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
PRKAA2 / AMPK Alpha 2 Antibody - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
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Mouse Monoclonal [clone 5B4G1] (IgG1) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IHC, WB
Unconjugated
100µl   $365.00
PRKAA2 / AMPK Alpha 2 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl   $285.00; 100µl   $385.00
PRKAA2 / AMPK Alpha 2 Antibody - Immunofluorescence analysis of A549 cells.
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
PRKAA2 / AMPK Alpha 2 Antibody
Select
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
ICC, IF, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $275.00
Select
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Mouse, Human
ELISA, IHC, WB
Unconjugated
100µg   0.25mg/ml   $485.00
PRKAA2 / AMPK Alpha 2 Antibody - Immunofluorescence staining of PRKAA2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PRKAA2 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Rat, Human
ICC, IF, IHC-P
Unconjugated, Preservative Free
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Mouse, Human
ELISA, IHC, WB
Unconjugated
100µg   $590.00
PRKAA2 / AMPK Alpha 2 Antibody - PRKAA2 antibody (1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Select
Goat Polyclonal to Human PRKAA2 / AMPK Alpha 2
Xenopus, Chicken, Bovine, Rat, Hamster, Pig, Horse, Bat, Rabbit, Mouse, Dog, Human, Monkey
ELISA, Peptide-ELISA, WB
Unconjugated
100µg   0.5mg/ml   $405.00
PRKAA2 / AMPK Alpha 2 Antibody - Fluorescent image of HeLa cell stained with PRKAA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). PRKAA2 immunoreactivity is localized to Cytoplasm significantly.
Select
Mouse Monoclonal (IgG2a,k) to Human PRKAA2 / AMPK Alpha 2
Human
IF, WB
Unconjugated
400µl   $325.00
Viewing 1-25 of 87 products



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).