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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRKAA2 / AMPK Alpha 2

protein kinase, AMP-activated, alpha 2 catalytic subunit

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Gene Name: protein kinase, AMP-activated, alpha 2 catalytic subunit
Family/Subfamily: Protein Kinase , AMPK/SNF1
Synonyms: PRKAA2, ACACA kinase, Alpha2 AMPK, AMPK alpha 2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, AMPK-2, AMPKa2, AMPKalpha2, Ampk alpha2, AMPK2, PRKAA, AMPK, AMPK-alpha-2 chain, HMGCR kinase
Target Sequences: NM_006252 NP_006243.2 P54646

Filtered by: None
Target
PRKAA2 / AMPK Alpha 2 (89)
Reactivity
Human (87)
Mouse (30)
Rat (24)
Bat (6)
Bovine (6)
Chicken (5)
Chimpanzee (3)
Dog (5)
Gibbon (3)
Guinea pig (4)
Hamster (2)
Horse (5)
Monkey (7)
Orangutan (3)
Pig (14)
Rabbit (6)
Sheep (4)
Xenopus (5)
Zebrafish (4)
Application
IHC (30)
IHC-P (6)
WB (84)
Flo (9)
ELISA (51)
ICC (2)
IF (8)
IP (3)
Peptide-ELISA (2)
RNAi (1)
Host
rabbit (74)
mouse (12)
goat (3)
Isotype
IgG (59)
IgG1 (2)
IgG2a,k (9)
Clonality
monoclonal mc (11)
polyclonal pc (78)
Clone
1G8 (1)
206CT5.4.2 (7)
5B4G1 (1)
8E11H5 (1)
Format
AP Conjugated (5)
APC (1)
APC Conjugated (6)
Azide-free (4)
Biotin Conjugated (9)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (8)
HRP Conjugated (8)
PE Conjugated (6)
Unconjugated (45)
Epitope
Thr172 (8)
aa252-493 (8)
aa453-483 (8)
aa476-507 (8)
C-Terminus (4)
aa323-372 (4)
Internal (1)
aa29-510 (1)
aa343-552 (1)
aa350-400 (1)
aa353-367 (1)
aa382-392 (1)
internal region (1)
pSer175 (1)
Publications
No (89)
Showing 1-25 of 89 results

PRKAA2 / AMPK Alpha 2 Antibody (phospho-Ser175) LS-C46806
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, WB
Unconjugated
100µg 0.5mg/ml  $275.00

PRKAA2 / AMPK Alpha 2 Antibody (clone 8E11H5) LS-C682437
Mouse Monoclonal [clone 8E11H5] (IgG1) to Human PRKAA2 / AMPK Alpha 2
Human, Monkey
ELISA, Flo, IHC, WB
Unconjugated
100µl  $365.00
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.

PRKAA2 / AMPK Alpha 2 Antibody (Thr172) LS-C324292
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
Unconjugated
200µl  $590.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, Biotin) LS-C216935
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
Biotin Conjugated
200µl  $740.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, FITC) LS-C216936
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
FITC Conjugated
200µl  $740.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, AP) LS-C216933
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
AP Conjugated
200µl  $740.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, APC) LS-C216934
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
APC Conjugated
200µl  $740.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, PE) LS-C216940
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
PE Conjugated
200µl  $740.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172, Azide-free, HRP) LS-C216937
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, Flo, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483) LS-C326836
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

PRKAA2 / AMPK Alpha 2 Antibody (aa453-483) LS-C100607
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PRKAA2 (arrow) using rabbit polyclonal PRKAA2 Antibody (RB11657). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2) (Origene Technologies).

PRKAA2 / AMPK Alpha 2 Antibody (aa29-510) LS-C185542
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ICC, IHC, IHC-P, IP, WB
Unconjugated
100µl 0.36mg/ml  $355.00
IHC of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using AMPK alpha 2 antibody at 1:200 dilution.

PRKAA2 / AMPK Alpha 2 Antibody LS-C679160
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:100
Immunofluorescent analysis of MCF7 cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PRKAA2 / AMPK Alpha 2 Antibody (clone 1G8) LS-C197983
Mouse Monoclonal [clone 1G8] (IgG2a,k) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IF, RNAi, WB
Unconjugated
100µg  $365.00
Immunofluorescence of monoclonal antibody to PRKAA2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of PRKAA2 expression in transfected 293T cell line by PRKAA2 monoclonal antibody (M02), clone 1G8.Lane 1: PRKAA2 transfected lysate(62.3 KDa).Lane 2: Non-transfected lysate.

PRKAA2 / AMPK Alpha 2 Antibody LS-C659577
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg  $405.00

PRKAA2 / AMPK Alpha 2 Antibody (Thr172) LS-C162143
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
PRKAA2 (Thr172) antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.This data demonstrates the use of the PRKAA2 (Thr172) antibody for immunohistochemistry.
PRKAA2 (Thr172) Antibody western blot of K562 cell line lysates (35 ug/lane). The PRKAA2 antibody detected the PRKAA2 protein (arrow).

PRKAA2 / AMPK Alpha 2 Antibody (clone 5B4G1) LS-C682438
Mouse Monoclonal [clone 5B4G1] (IgG1) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IHC, WB
Unconjugated
100µl  $365.00
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PRKAA2 mouse mAb with DAB staining.

PRKAA2 / AMPK Alpha 2 Antibody (aa343-552) LS-C378047
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot. All lanes: PRKAA2 antibody at 8 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa.

PRKAA2 / AMPK Alpha 2 Antibody LS-C349072
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of A549 cells.
Western blot analysis of extracts of various cell lines.

PRKAA2 / AMPK Alpha 2 Antibody LS-C668665
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Human
ICC, IF, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $275.00
Immunofluorescence Image
Western blot Image

PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) LS-C122646
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Mouse, Human
ELISA, IHC, WB
Unconjugated
100µg  $590.00

PRKAA2 / AMPK Alpha 2 Antibody LS-C146865
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Mouse, Human
ELISA, IHC, WB
Unconjugated
100µg  $485.00

PRKAA2 / AMPK Alpha 2 Antibody (Internal) LS-C155350
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $355.00
IHC of paraffin-embedded Colon carcinoma, using AMPK alpha 2 antibody at 1:500 dilution.
IHC of paraffin-embedded mouse brain, using AMPK alpha 2 antibody at 1:500 dilution.

PRKAA2 / AMPK Alpha 2 Antibody LS-C162144
Mouse Monoclonal (IgG2a,k) to Human PRKAA2 / AMPK Alpha 2
Human
IF, WB
Unconjugated
400µl  $325.00
Fluorescent image of HeLa cell stained with PRKAA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). PRKAA2 immunoreactivity is localized to Cytoplasm significantly.
PRKAA2 antibody western blot of CEM cell line lysates (35 ug/lane). The PRKAA2 antibody detected the PRKAA2 protein (arrow).

PRKAA2 / AMPK Alpha 2 Antibody (aa382-392) LS-C139628
Goat Polyclonal to Human PRKAA2 / AMPK Alpha 2
Xenopus, Chicken, Bovine, Rat, Hamster, Pig, Horse, Bat, Rabbit, Mouse, Dog, Human, Monkey
ELISA, Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
PRKAA2 antibody (1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PRKAA2 Antibody (0.3µg/ml) staining of Mouse (A) and Rat (B) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
Showing 1-25 of 89 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).