PRKAA1 / AMPK Alpha 1

protein kinase, AMP-activated, alpha 1 catalytic subunit

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

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Products

ELISA Kits (20)

Type

Cell-Based (1)

Cell-Based Phosphorylation (1)

Competitive EIA (1)

Custom (3)

Sandwich (13)

Sandwich CLIA (1)

Target

PRKAA1 / AMPK Alpha 1 (20)

Reactivity

Human (7)

Mouse (5)

Rat (5)

Chicken (1)

Guinea pig (1)

Monkey (1)

Pig (1)

Porcine (1)

Rabbit (1)

Sheep (1)

Format

12 x 8-Well Microstrips (1)

96-Well Microplate (2)

96-Well Strip Plate (17)

Publications

No (20)

Sample Type

Adherent Cell Cultures (2)

Ascites (1)

Cell Culture Supernatants (1)

Cell Lysates (3)

Cerebrospinal Fluid (1)

Plasma (6)

Saliva (1)

Serum (6)

Tissue Homogenates (5)

Urine (1)

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Human PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F27161

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.5 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Ascites, Cell Culture Supernatants, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenates, Urine

Price:

1 Plate/$913

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Guinea pig PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F47422

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Guinea pig

Range:

3.906 - 250 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Chicken PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F47421

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Chicken

Range:

3.906 - 250 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Porcine PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F47424

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Porcine

Range:

78.125 - 5000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Monkey PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F47423

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Monkey

Range:

78.125 - 5000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Sheep PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F47426

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Sheep

Range:

39.063 - 2500 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$760

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Rabbit PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F47425

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rabbit

Range:

3.125 - 200 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Rat PRKAA1 / AMPK Alpha 1 (Custom ELISA) ELISA Kit - LS-F20540

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Rat

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$783

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Mouse PRKAA1 / AMPK Alpha 1 (Custom ELISA) ELISA Kit - LS-F20541

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Mouse

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$783

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Pig PRKAA1 / AMPK Alpha 1 (Custom ELISA) ELISA Kit - LS-F20542

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Pig

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$783

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Human PRKAA1 / AMPK Alpha 1 (CLIA) ELISA Kit - LS-F29296

Type:

Sandwich CLIA

Format:

12 x 8-Well Microstrips

Reactivity:

Human

Range:

62.5 - 4000 pg/ml

Detection:

Chemiluminescent

Sample Types:

Plasma, Serum

Price:

1 Plate/$728

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Mouse PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F25223

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

1.56 - 100 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$782

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Human PRKAA1 / AMPK Alpha 1 (Competitive EIA) ELISA Kit - LS-F39185

Type:

Competitive EIA

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

78 - 5000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

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Human PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F37875

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

78.125 - 5000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

Select

Mouse PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F38402

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

1.563 - 100 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

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Rat PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F38689

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rat

Range:

3.125 - 200 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

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Rat PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F55289

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rat

Range:

1.56 - 100 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$818

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Human PRKAA1 / AMPK Alpha 1 (Sandwich ELISA) ELISA Kit - LS-F9281

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$764

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Mouse/Human/Rat Phospho-PRKAA1 / AMPK Alpha 1 (Ser485) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1449

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

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Mouse/Human/Rat PRKAA1 / AMPK Alpha 1 (Cell-Based ELISA) ELISA Kit - LS-F1794

Type:

Cell-Based

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$618

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