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PNP / Nucleoside Phosphorylase

purine nucleoside phosphorylase

Nucleoside Phosphorylase / PNP is an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

Gene Name: purine nucleoside phosphorylase
Synonyms: PNP, Inosine phosphorylase, NP, Nucleoside phosphorylase, PUNP, PRO1837
Target Sequences: NM_000270 NP_000261.2 P00491

Publications (1)

1
Partial Purine Nucleoside Phosphorylase Deficiency Helps Determine Minimal Activity Required for Immune and Neurological Development. Eyal Grunebaum, Nicholas Campbell, Matilde Leon-Ponte, Xiaobai Xu, Hugo Chapdelaine. Frontiers in immunology. 2020 July;11:1257. [Full Text Article] [PubMed:32695102] [PMC:PMC7338719] Related Antibodies: LS-C137543.

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Over-Expression Lysate (2)
Recombinant (8)
PNP / Nucleoside Phosphorylase (10)
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GST (1)
His (2)
His-SUMO (1)
His-T7, N-terminus (3)
Myc-DDK (Flag) (3)
Human (7)
Mouse (1)
Rat (1)
293T Cells (1)
E. coli (6)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (4)
PNP / Nucleoside Phosphorylase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
31.9 kDa
100 µg/$494
PNP / Nucleoside Phosphorylase Protein - Recombinant Nucleoside Phosphorylase (NP) by SDS-PAGE
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E. coli
His-T7, N-terminus
36.0kDa
10 µg/$274; 50 µg/$395; 200 µg/$790; 100 µg/$632; 1 mg/$1,858; 500 µg/$1,414; 5 mg/$2,820; 2 mg/$2,041
PNP / Nucleoside Phosphorylase Protein - 12.5% SDS-PAGE of human NP stained with Coomassie Blue
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Wheat Germ Extract
GST
58.5 kDa
10 µg/$479; 25 µg/$670
PNP / Nucleoside Phosphorylase Protein
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E. coli
His-SUMO
48.1 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
PNP / Nucleoside Phosphorylase Protein - Purified recombinant protein PNP was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
31.9 kDa
20 µg/$1,107
PNP / Nucleoside Phosphorylase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
31.9 kDa
20 µg/$150
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E. coli
His
33.5 kDa
50 µg/$854
PNP / Nucleoside Phosphorylase Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
PNP / Nucleoside Phosphorylase Protein - Recombinant Nucleoside Phosphorylase (NP) by SDS-PAGE
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E. coli
His-T7, N-terminus
35.7kDa
10 µg/$274; 50 µg/$395; 200 µg/$790; 100 µg/$632; 1 mg/$1,858; 500 µg/$1,414; 5 mg/$2,820; 2 mg/$2,041
PNP / Nucleoside Phosphorylase Protein - Recombinant Nucleoside Phosphorylase (NP) by SDS-PAGE
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E. coli
His-T7, N-terminus
35.8kDa
10 µg/$269; 50 µg/$365; 200 µg/$730; 100 µg/$582; 1 mg/$1,766; 500 µg/$1,335; 5 mg/$2,722; 2 mg/$1,947
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).