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PLOD / PLOD1

procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Gene Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
Synonyms: PLOD1, LLH, Lysyl hydroxlase 1, PLOD, LH, LH1, Lysine hydroxylase, Lysyl hydroxylase 1, EDS6
Target Sequences: NM_000302 NP_000293.2 Q02809

Publications (1)

1
Defining the Extracellular Matrix of Rhabdomyosarcoma. Xiaolei Lian, J Steffan Bond, Narendra Bharathy, Sergei P Boudko, Elena Pokidysheva, Jack F Shern, Melvin Lathara, Takako Sasaki, Teagan Settelmeyer, Megan M Cleary, Ayeza Bajwa, Ganapati Srinivasa, Christopher P Hartley, Hans Peter Bächinger, Atiya Mansoor, Sakir H Gultekin, Noah E Berlow, Charles Keller. Frontiers in oncology. 2021 March;11:601957. [Full Text Article] [PubMed:33708626] [PMC:PMC7942227] Related Antibodies: LS-C163796.

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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
PLOD / PLOD1 (6)
No (6)
GST (1)
GST, N-terminus (1)
His (1)
Myc-DDK (Flag) (3)
Human (5)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (1)
PLOD / PLOD1 Protein - 12.5% SDS-PAGE of human PLOD1 stained with Coomassie Blue
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Wheat Germ Extract
GST
109.9 kDa
2 µg/$439
PLOD / PLOD1 Protein - Recombinant Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 By SDS-PAGE
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E. coli
His
14.2 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
PLOD / PLOD1 Protein - Purified recombinant protein PLOD1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
81.5 kDa
20 µg/$1,107
PLOD / PLOD1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
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293T Cells
Myc-DDK (Flag)
83.6 kDa
20 µg/$150
PLOD / PLOD1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
83.6 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).