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PKM / Pyruvate Kinase, Muscle

pyruvate kinase, muscle

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Gene Name: pyruvate kinase, muscle
Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1
Target Sequences: NM_002654 NP_002645.3 P14618

Publications (2)

1
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL. Cell. 2011 145:732-44. (WB, ChrIP; Human) [PubMed:21620138] [PMC:PMC3130564]
2
Enhancing mitochondrial respiration suppresses tumor promoter TPA-induced PKM2 expression and cell transformation in skin epidermal JB6 cells. Wittwer JA, Robbins D, Wang F, Codarin S, Shen X, Kevil CG, Huang TT, Van Remmen H, Richardson A, Zhao Y. Cancer prevention research (Philadelphia, Pa.). 2011 4:1476-84. [PubMed:21673231]

☰ Filters
Products
Proteins (22)
Over-Expression Lysate (6)
Recombinant (16)
PKM / Pyruvate Kinase, Muscle (22)
No (22)
10His, N-terminus + Myc, C-terminus (4)
6His, N-terminus (2)
GST (2)
GST, N-terminus (1)
His (2)
His-GST (1)
His-T7 (1)
Myc-DDK (Flag) (9)
Human (14)
Mouse (2)
Rat (3)
293T Cells (3)
Baculovirus (2)
E. coli (8)
HEK 293 Cells (6)
Wheat Germ Extract (3)
Purified (6)
Low endotoxin level (2)
Biologically active (1)
PKM / Pyruvate Kinase, Muscle Protein
Select
E. coli
His
Biologically active
100 µg/$342; 500 µg/$641; 20 µg/$254
PKM / Pyruvate Kinase, Muscle Protein - Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of Recombinant Rat Pyruvate kinase PKM(Pkm) could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Pkm.
Select
E. coli
10His, N-terminus + Myc, C-terminus
62.8 kDa
20 µg/$405; 1 mg/$1,679; 100 µg/$598
PKM / Pyruvate Kinase, Muscle Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
Baculovirus
10His, N-terminus + Myc, C-terminus
61.8 kDa
100 µg/$1,164; 20 µg/$475; 1 mg/$2,187
PKM / Pyruvate Kinase, Muscle Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
Baculovirus
10His, N-terminus + Myc, C-terminus
61.8 kDa
100 µg/$1,164; 20 µg/$475; 1 mg/$2,187
PKM / Pyruvate Kinase, Muscle Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
10His, N-terminus + Myc, C-terminus
62.8 kDa
1 mg/$1,679; 100 µg/$598; 20 µg/$405
PKM / Pyruvate Kinase, Muscle Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
PKM / Pyruvate Kinase, Muscle Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
57.9 kDa
100 µg/$710
PKM / Pyruvate Kinase, Muscle Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
57.9 kDa
100 µg/$710
PKM / Pyruvate Kinase, Muscle Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
57.8 kDa
100 µg/$494
PKM / Pyruvate Kinase, Muscle Protein - 12.5% SDS-PAGE of human PKM2 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
84.15 kDa
10 µg/$479; 25 µg/$670
PKM / Pyruvate Kinase, Muscle Protein - 12.5% SDS-PAGE of human PKM2 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
84.15 kDa
2 µg/$439
PKM / Pyruvate Kinase, Muscle Protein - Purified recombinant protein PKM was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
57.8 kDa
20 µg/$1,107
PKM / Pyruvate Kinase, Muscle Protein - Purified recombinant protein PKM was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
57.9 kDa
20 µg/$1,107
PKM / Pyruvate Kinase, Muscle Protein - Purified recombinant protein PKM was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
57.9 kDa
20 µg/$1,107
Select
293T Cells
Myc-DDK (Flag)
57.8 kDa
20 µg/$150
PKM / Pyruvate Kinase, Muscle Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
57.9 kDa
20 µg/$215
PKM / Pyruvate Kinase, Muscle Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
57.9 kDa
20 µg/$215
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
60.1 kDa
10 µg/$350; 50 µg/$440
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
60.1kD
10 µg/$262; 50 µg/$354; 500 µg/$905; 1 mg/$1,203
PKM / Pyruvate Kinase, Muscle Protein - Recombinant Pyruvate Kinase, Muscle By SDS-PAGE
Select
E. coli
His
13.4 kDa
10 µg/$284; 50 µg/$462; 100 µg/$737; 200 µg/$922; 1 mg/$2,042; 500 µg/$1,577; 5 mg/$3,221; 2 mg/$2,231
PKM / Pyruvate Kinase, Muscle Protein - Recombinant Pyruvate Kinase, Muscle By SDS-PAGE
Select
E. coli
His-GST
51.2 kDa
10 µg/$284; 50 µg/$462; 100 µg/$737; 200 µg/$922; 1 mg/$2,042; 500 µg/$1,577; 5 mg/$3,221; 2 mg/$2,231
PKM / Pyruvate Kinase, Muscle Protein - Recombinant Pyruvate Kinase, Muscle By SDS-PAGE
Select
E. coli
His-T7
26.4 kDa
10 µg/$287; 50 µg/$475; 100 µg/$760; 200 µg/$950; 1 mg/$2,078; 500 µg/$1,609; 5 mg/$3,319; 2 mg/$2,268
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).