PKG / PRKG1

protein kinase, cGMP-dependent, type I

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca2+ release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca2+ levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

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Proteins (9)

Type

Over-Expression Lysate (4)

Recombinant (5)

Target

PKG / PRKG1 (9)

Publications

No (9)

Tag

6His, C-terminus (2)

GST (1)

GST, N-terminus (1)

Myc-DDK (Flag) (5)

Species

Human (7)

Source

293T Cells (2)

HEK 293 Cells (3)

Human Cells (1)

Mammalian Cells (1)

Wheat Germ Extract (2)

Purification

Purified (2)

Low Endotoxin Level

Low endotoxin level (2)

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PKG / PRKG1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G115429

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

77.6 kDa

Price:

100 µg/$710

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Human PKG / PRKG1 Protein (Recombinant 6His, C-terminus) (Gly2-Pro686) - LS-G140102

Source:

Mammalian Cells

Tag:

6His, C-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

78.8 kDa

Price:

10 µg/$344; 50 µg/$584

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PKG / PRKG1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G119238

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

76.2 kDa

Price:

100 µg/$710

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Human PKG / PRKG1 Protein (Recombinant GST) (Full Length) - LS-G29910

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

101.09 kDa

Price:

10 µg/$479; 25 µg/$670

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Human PKG / PRKG1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75149

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

77.6 kDa

Price:

20 µg/$215

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Human PKG / PRKG1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75148

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

76.2 kDa

Price:

20 µg/$215

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Human PKG / PRKG1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G65242

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

77.6 kDa

Price:

20 µg/$1,107

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Human PKG / PRKG1 Protein (Recombinant GST, N-terminus) (aa73-170) - LS-G59739

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human PKG / PRKG1 Protein (Recombinant 6His, C-terminus) (Gly2-Pro686) - LS-G39239

Source:

Human Cells

Tag:

6His, C-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

78.8kD

Price:

10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157

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