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PGK1 / Phosphoglycerate Kinase

phosphoglycerate kinase 1

PGK1/Phosphoglycerate Kinase 1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Gene Name: phosphoglycerate kinase 1
Family/Subfamily: Non-protein Kinase , PGK
Synonyms: PGK1, MIG10, Primer recognition protein 2, PRP 2, PGKA, Phosphoglycerate kinase 1
Target Sequences: NM_000291 NP_000282.1 P00558

Publications (4)

1
Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance. Kilic M, Kasperczyk H, Fulda S, Debatin KM. Oncogene. 2007 26:2027-38. [PubMed:17043658] Related Antibodies: LS-C98055.
2
Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice. Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, O'Brien DA. Biology of reproduction. 2010 82:136-45. [PubMed:19759366] [PMC:PMC2802118] Related Antibodies: LS-C98055.
3
Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach. Fedorova LV, Tamirisa A, Kennedy DJ, Haller ST, Budnyy G, Shapiro JI, Malhotra D. BMC nephrology. 2013 14:209. (WB; Human) [Full Text Article] [PubMed:24090408] Related Antibodies: LS-B5809.
4
HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Ekman M, Uvelius B, Albinsson S, Sw[Character e4]rd K. Laboratory investigation; a journal of technical methods and pathology. May;94:557-68. [Full Text Article] [PubMed:24589856] Related Antibodies: LS-B5809.
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☰ Filters
Products
Proteins (13)
Over-Expression Lysate (2)
Recombinant (11)
PGK1 / Phosphoglycerate Kinase (13)
No (13)
6His, C-terminus (2)
GST (2)
GST, N-terminus (1)
His (4)
His,C-terminus (1)
Myc-DDK (Flag) (3)
Human (10)
Mouse (2)
293T Cells (1)
E. coli (5)
HEK 293 Cells (2)
Human Cells (1)
Insect Cells (1)
Mammalian Cells (1)
Wheat Germ Extract (2)
Purified (3)
Low endotoxin level (5)
PGK1 / Phosphoglycerate Kinase Protein
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$361; 500 µg/$739; 20 µg/$261
PGK1 / Phosphoglycerate Kinase Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
PGK1 / Phosphoglycerate Kinase Protein - 12.5% SDS-PAGE of human PGK1 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
71 kDa
10 µg/$479; 25 µg/$670
Select
E. coli
GST
69.3 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
PGK1 / Phosphoglycerate Kinase Protein - Recombinant Phosphoglycerate Kinase 1 By SDS-PAGE
Select
E. coli
His
48.2 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
PGK1 / Phosphoglycerate Kinase Protein - Purified recombinant protein PGK1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.4 kDa
20 µg/$1,107
Select
E. coli
416 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
45.87 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
Select
Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
45.5kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,353
Select
Insect Cells
His,C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
46 kDa
50 µg/$561
PGK1 / Phosphoglycerate Kinase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
44.4 kDa
100 µg/$494
PGK1 / Phosphoglycerate Kinase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
44.4 kDa
20 µg/$150
PGK1 / Phosphoglycerate Kinase Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
45.5 kDa
10 µg/$344; 50 µg/$584
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).