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PDGF-BB

platelet-derived growth factor beta polypeptide

PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Gene Name: platelet-derived growth factor beta polypeptide
Family/Subfamily: PDGF , not assigned-PDGF
Synonyms: PDGFB, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain
Target Sequences: NM_002608 NP_002599.1 P01127

Publications (5)

1
Effects of Increased Renal Tubular Vascular Endothelial Growth Factor (VEGF) on Fibrosis, Cyst Formation, and Glomerular Disease. Samy Hakroush, Marcus J. Moeller, Franziska Theilig, Brigitte Kaissling, Tjeerd P. Sijmonsma, Manfred Jugold, Ann L. Akeson, Milena Traykova-Brauch, Hiltraud Hosser, Brunhilde Hähnel, Hermann-Josef Gröne, Robert Koesters, and Wilhelm Kriz. The American journal of pathology. 2009 275:1883-95. (IHC-P; Mouse) [Full Text Article] [PubMed:19834063] [PMC:PMC2774053]
2
Severe renal mass reduction impairs recovery and promotes fibrosis after AKI. Polichnowski AJ, Lan R, Geng H, Griffin KA, Venkatachalam MA, Bidani AK. Journal of the American Society of Nephrology : JASN. 2014 25:1496-507. (WB; Human) [Full Text Article] [PubMed:24511135] [PMC:PMC4073422]
3
Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease. Ana M Porras, Jennifer A Westlund, Austin D Evans, Kristyn S Masters. Proceedings of the National Academy of Sciences of the United States of America. 2018 January;115:E363-E371. [Full Text Article] [PubMed:29282325] [PMC:PMC5776956] Related Antibodies: LS-B4182.
4
Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes. Jing Bai , Mehrdad Khajavi, Lufei Sui, Haojie Fu, Subrahmanian Tarakkad Krishnaji, Amy E Birsner, Lauren Bazinet, Roger D Kamm, Robert J D'Amato. Angiogenesis. 2021 Feb;;24:111-127. [Full Text Article] [PubMed:32955682] Related Antibodies: LS-C201038.
5
Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and G alpha q-mediated Rho and alpha v beta 6 integrin-dependent activation of TGF- beta . Geng H, Lan R, Singha PK, Gilchrist A, Weinreb PH, Violette SM, Weinberg JM, Saikumar P, Venkatachalam MA. The American journal of pathology. 2012 Oct;181:1236-49. [Full Text Article] [PubMed:22885106] [PMC:PMC3463629] Related Antibodies: LS-B4182.
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Products
Proteins (34)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (6)
Recombinant (27)
PDGF-BB (34)
Animal-free (1)
No (34)
6His, C-terminus (2)
6His, N-terminus (4)
GST, N-terminus (1)
His (1)
His, N-Terminal (2)
His, N-terminus (1)
His,C-terminus (1)
His,N-terminus (1)
His-GST (2)
His-S (1)
His-T7 (1)
Myc-DDK (Flag) (4)
Human (19)
Mouse (10)
Rat (3)
293T Cells (2)
E. coli (24)
HEK 293 Cells (5)
Wheat Germ Extract (1)
Yeast (2)
Purified (7)
Low endotoxin level (16)
Biologically active (12)
Animal-free (1)
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HEK 293 Cells
6His, N-terminus
15 kDa
100 µg/$298
PDGF-BB Protein
Select
E. coli
6His, N-terminus
12 kDa
10 µg/$265; 50 µg/$315; 100 µg/$365
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
24.4 kDa
2 µg/$268; 10 µg/$349
PDGF-BB Protein - Human PDGF-BB induces the proliferation of NIH 3T3 mouse cells.
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg (determined by LAL method).
The 109 amino acid recombinant protein has a predicted molecular mass of 12,294 Da. The DTT-reduced and the non-reduced protein migrate at approximately 13 and 37 KDa by SDS-PAGE respectively. The N-terminal amino acid is Serine.
10 µg/$306; 25 µg/$383; 100 µg/$900; 500 µg/$1,828
Select
E. coli
6His, C-terminus
Less than 0.1 ng/mL (Less than 1 EU/µg) (determined by LAL method).
10 µg/$288; 50 µg/$442; 500 µg/$1,654; 1 mg/$2,353
PDGF-BB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
25.3 kDa
100 µg/$494
PDGF-BB Protein
Select
E. coli
His, N-Terminal
24.9 kDa
50 µg/$419
PDGF-BB Protein - Recombinant Rat PDGF-B Protein (His Tag)-Elabscience
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E. coli
His, N-terminus
12.2 kDa
20 µg/$270; 100 µg/$390
Select
E. coli
None
Biologically active
Less than 1 EU/µg (determined by LAL method).
24.8 kDa
10 µg/$364; 1 mg/$3,412
Select
Yeast
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
24.3 kDa (SDS-PAGE)
10 µg/$304; 50 µg/$466; 1 mg/$2,653
Select
HEK 293 Cells
6His, N-terminus
15 kDa
200 µl/$283
PDGF-BB Protein - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Select
E. coli
6His, N-terminus
14 kDa
10 µg/$265; 50 µg/$315; 100 µg/$365
Select
HEK 293 Cells
His, N-Terminal
~12 kDa
100 µg/$298
PDGF-BB Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
PDGF-BB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
27.28 kDa
20 µg/$150
PDGF-BB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
25.3 kDa
20 µg/$150
PDGF-BB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
27.28 kDa
100 µg/$494
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
24.3 kDa
2 µg/$268; 10 µg/$349
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
2 µg/$281; 10 µg/$369
PDGF-BB Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
12.42 kDa
50 µg/$698; 10 µg/$356; 1 mg/$2,837; 500 µg/$1,987
PDGF-BB Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His, C-terminus
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
13.4 kDa
50 µg/$516; 10 µg/$313; 1 mg/$3,095; 500 µg/$2,115
PDGF-BB Protein - NIH/3T3 cell proliferation induced by mouse PDGF-BB.
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg (determined by LAL method).
The 109 amino acid recombinant protein has a predicted molecular mass of approximately 12.2 kD. The DTT-reduced and non-reduced protein migrate at approximately 14 and 38 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ser.
10 µg/$306; 25 µg/$383; 100 µg/$900
Select
E. coli
His,C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
13.4 kDa
10 µg/$288; 50 µg/$436
PDGF-BB Protein
Select
E. coli
His
50 µg/$411; 250 µg/$985; 10 µg/$276
Select
Yeast
His,N-terminus
14.3 kDa
50 µg/$793; 1 mg/$3,773
Viewing 1-25 of 34 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).