OTUB1 / OTU1

OTU domain, ubiquitin aldehyde binding 1

Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.

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Proteins (10)

Type

Over-Expression Lysate (2)

Recombinant (8)

Target

OTUB1 / OTU1 (10)

Publications

No (10)

Tag

GST (2)

His (5)

Myc-DDK (Flag) (3)

Species

Human (7)

Mouse (1)

Rat (1)

Source

293T Cells (1)

E. coli (6)

HEK 293 Cells (2)

Wheat Germ Extract (1)

Purification

Purified (3)

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Human OTUB1 / OTU1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G90592

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

31.1 kDa

Price:

20 µg/$150

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Human OTUB1 / OTU1 Protein (Recombinant His) (aa1-271) - LS-G16175

Source:

E. coli

Tag:

His

Molecular Weight:

32.5 kDa

Price:

10 µg/$268; 50 µg/$362; 100 µg/$577; 200 µg/$722; 1 mg/$1,755; 500 µg/$1,325; 5 mg/$2,708; 2 mg/$1,934

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Human OTUB1 / OTU1 Protein (Recombinant GST) (Full Length) - LS-G22218

Source:

E. coli

Tag:

GST

Molecular Weight:

50.5 kD

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

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Human OTUB1 / OTU1 Protein (Recombinant GST) (Full Length) - LS-G34354

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

55.55 kDa

Price:

10 µg/$479; 25 µg/$670

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Human OTUB1 / OTU1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G71775

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

31.1 kDa

Price:

20 µg/$1,107

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OTUB1 / OTU1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G101491

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

31.1 kDa

Price:

100 µg/$494

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Human OTUB1 / OTU1 Protein (Recombinant His) (Met1-Lys271) - LS-G97742

Source:

E. coli

Tag:

His

Molecular Weight:

32.8 kDa

Price:

100 µg/$451

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Human OTUB1 / OTU1 Protein (Recombinant His) - LS-G575

Source:

E. coli

Tag:

His

Price:

100 µg/$361; 500 µg/$739; 20 µg/$261

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Rat OTUB1 / OTU1 Protein (Recombinant His) (aa1-271) - LS-G16172

Source:

E. coli

Tag:

His

Molecular Weight:

32.5 kDa

Price:

10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060

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Mouse OTUB1 / OTU1 Protein (Recombinant His) (aa1-271) - LS-G16173

Source:

E. coli

Tag:

His

Molecular Weight:

32.5 kDa

Price:

10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119

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