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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ONECUT1 / HNF6

one cut homeobox 1

ONECUT1 / HNF6 is a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. Alternative splicing results in multiple transcript variants.

Gene Name: one cut homeobox 1
Synonyms: ONECUT1, Hepatocyte nuclear factor 6, HNF6, One cut domain family member 1, One cut homeobox 1, HNF-6, HNF6A
Target Sequences: U77975 AAB61705.1 Q9UBC0

Filtered by: None
Target
ONECUT1 / HNF6 (34)
Reactivity
Human (33)
Mouse (10)
Rat (13)
Bat (13)
Bovine (8)
Chicken (4)
Chimpanzee (9)
Dog (13)
Gibbon (9)
Guinea pig (8)
Horse (8)
Monkey (10)
Pig (12)
Rabbit (12)
Xenopus (4)
Zebrafish (8)
Application
IHC (11)
IHC-Fr (1)
IHC-P (8)
WB (31)
ELISA (11)
ICC (1)
IF (2)
Host
rabbit (32)
mouse (2)
Isotype
IgG (24)
IgG1 (2)
Clonality
monoclonal mc (2)
polyclonal pc (32)
Clone
OTI4F12 (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (5)
Carrier-free (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (1)
Unconjugated (16)
Epitope
aa356-385 (8)
C-Terminus (4)
N-Terminus (4)
aa370-419 (4)
aa187-203 (1)
aa420-470 (1)
aa85-198 (1)
Publications
No (34)
Showing 1-25 of 34 results

IHC-plus™ ONECUT1 / HNF6 Antibody (C-Terminus) LS-B3525
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Zebrafish, Pig, Horse, Bat, Rabbit, Dog, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Anti-ONECUT1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ONECUT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ONECUT1 / HNF6 Antibody (C-Terminus, HRP) LS-C436958
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Zebrafish, Rat, Rabbit, Dog, Pig, Horse, Bat, Gibbon, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
HRP Conjugated
100µl 0.65mg/ml  $405.00
Anti-ONECUT1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ONECUT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ONECUT1 / HNF6 Antibody (C-Terminus, Biotin) LS-C436956
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Zebrafish, Rat, Rabbit, Dog, Pig, Horse, Bat, Gibbon, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
Biotin Conjugated
100µl 1mg/ml  $405.00
Anti-ONECUT1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ONECUT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ONECUT1 / HNF6 Antibody (C-Terminus, FITC) LS-C436957
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Zebrafish, Rat, Rabbit, Dog, Pig, Horse, Bat, Gibbon, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
FITC Conjugated
100µl 1mg/ml  $405.00
Anti-ONECUT1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ONECUT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ONECUT1 / HNF6 Antibody (aa187-203) LS-C313311
Rabbit Polyclonal to Human ONECUT1 / HNF6
Mouse, Rat, Bat, Human, Monkey
ICC, IHC, IHC-Fr, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
ONECUT1 / HNF6 antibody. IHC(P): Human Liver Cancer Tissue.
ONECUT1 / HNF6 antibody. IHC(P): Rat Liver Tissue.

ONECUT1 / HNF6 Antibody (clone OTI4F12) LS-C114401
Mouse Monoclonal [clone OTI4F12] (IgG1) to Human ONECUT1 / HNF6
IF, IHC, IHC-P, WB
Unconjugated
100µl 0.72mg/ml  $345.00
Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-HNF6 mouse monoclonal antibody.
Anti-HNF6 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6.

ONECUT1 / HNF6 Antibody (aa85-198, clone OTI4F12, Carrier-free) LS-C784129
Mouse Monoclonal [clone OTI4F12] (IgG1) to Human ONECUT1 / HNF6
Human
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-HNF6 mouse monoclonal antibody.
Anti-HNF6 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6.

ONECUT1 / HNF6 Antibody LS-C672053
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Positive Western Blot detected in Jurkat whole cell lysate, Rat brain tissue. All lanes: ONECUT1 antibody at 7.6 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size:52 KDa. Observed band size: 52 KDa

ONECUT1 / HNF6 Antibody LS-C667357
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, IHC, WB
Unconjugated
100µg  $355.00

ONECUT1 / HNF6 Antibody (aa420-470) LS-C148720
Rabbit Polyclonal to Human ONECUT1 / HNF6
Dog, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µg  $335.00
IHC of HNF6 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and Peptide-affinity Purified Polyclonal Antibody to HNF6 (bottom) at 5 ug/ml.
Western blot of HNF6 in human pancreas in the 1) absence and 2) presence of immunizing peptide, and 3) rat pancreas using Peptide-affinity Purified Polyclonal Antibody to HNF6 at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.

ONECUT1 / HNF6 Antibody LS-C411202
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Mouse, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00

ONECUT1 / HNF6 Antibody (aa356-385) LS-C323527
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
Unconjugated
200µl  $590.00

ONECUT1 / HNF6 Antibody (aa356-385, Biotin) LS-C216176
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

ONECUT1 / HNF6 Antibody (aa356-385, FITC) LS-C216177
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

ONECUT1 / HNF6 Antibody (aa356-385, AP) LS-C216174
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
AP Conjugated
200µl  $740.00

ONECUT1 / HNF6 Antibody (aa356-385, APC) LS-C216175
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
APC Conjugated
200µl  $740.00

ONECUT1 / HNF6 Antibody (aa356-385, Azide-free, HRP) LS-C216178
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

ONECUT1 / HNF6 Antibody (aa356-385, PE) LS-C216179
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Human
ELISA, WB
PE Conjugated
200µl  $740.00

ONECUT1 / HNF6 Antibody LS-C781593
Rabbit Polyclonal to Human ONECUT1 / HNF6
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg 100µl 1mg/ml  $305.00

ONECUT1 / HNF6 Antibody LS-C809176
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Western blot Image

ONECUT1 / HNF6 Antibody (aa370-419) LS-C145424
Rabbit Polyclonal to Mouse ONECUT1 / HNF6
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
ONECUT1 antibody Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ONECUT1 / HNF6 Antibody LS-C768726
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Mouse, Rat, Human
WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

ONECUT1 / HNF6 Antibody (N-Terminus) LS-C101697
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Rabbit, Dog, Guinea pig, Bovine, Pig, Bat, Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
ONECUT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

ONECUT1 / HNF6 Antibody LS-C747848
Rabbit Polyclonal (IgG) to Human ONECUT1 / HNF6
Mouse, Rat, Human
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using ONECUT1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

ONECUT1 / HNF6 Antibody (aa356-385) LS-C163122
Rabbit Polyclonal to Human ONECUT1 / HNF6
Human
WB
Unconjugated
400µl  $325.00
ONECUT1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ONECUT1 antibody detected the ONECUT1 protein (arrow).
Showing 1-25 of 34 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).