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NMNAT1 / NMNAT

nicotinamide nucleotide adenylyltransferase 1

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, prefers NAD+ and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following mechanical or toxic insults.

Gene Name: nicotinamide nucleotide adenylyltransferase 1
Synonyms: NMNAT1, LCA9, NMN adenylyltransferase 1, NMNAT, PNAT1, NaMN adenylyltransferase 1
Target Sequences: NM_022787 NP_073624.2 Q9HAN9

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Products
Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
NMNAT1 / NMNAT (9)
No (9)
GST (1)
GST, N-terminus (2)
His (3)
Myc-DDK (Flag) (3)
Human (8)
293T Cells (1)
Baculovirus (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (3)
Low endotoxin level (1)
Biologically active (1)
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E. coli
His
Biologically active
~33.3kDa (monomer)
50 µg/$456; 10 µg/$344
NMNAT1 / NMNAT Protein - 12.5% SDS-PAGE of human NMNAT1 stained with Coomassie Blue
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Wheat Germ Extract
GST
58.3 kDa
2 µg/$439
NMNAT1 / NMNAT Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
NMNAT1 / NMNAT Protein - Purified recombinant protein NMNAT1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
31.8 kDa
20 µg/$1,107
NMNAT1 / NMNAT Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
GST, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
NMNAT1 / NMNAT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
31.8 kDa
20 µg/$150
Select
Baculovirus
His
Less than 1.0 EU/µg protein (determined by LAL method).
33.3 kDa
50 µg/$854
NMNAT1 / NMNAT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
31.8 kDa
100 µg/$494
NMNAT1 / NMNAT Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).