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NAMPT / Visfatin

nicotinamide phosphoribosyltransferase

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.

Gene Name: nicotinamide phosphoribosyltransferase
Synonyms: NAMPT, PBEF, Pre-B cell-enhancing factor, VISFATIN, VF, NAmPRTase, PBEF1
Target Sequences: NM_005746 NP_005737.1 P43490

Publications (4)

1
Monomeric eNAMPT in the development of experimental diabetes in mice: a potential target for type 2 diabetes treatment. Kieswich J, Sayers SR, Silvestre MF, Harwood SM, Yaqoob MM, Caton PW. Diabetologia. 2016 59:2477-2486. (WB; Mouse) [Full Text Article] [PubMed:27541013] [PMC:PMC5506101]
2
Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Duo Feng, DongZhu Xu, Nobuyuki Murakoshi, Kazuko Tajiri, Rujie Qin, Saori Yonebayashi, Yuta Okabe, Siqi Li, Zixun Yuan, Kazutaka Aonuma, Masaki Ieda. International journal of molecular sciences. 2020 June;21: [Full Text Article] [PubMed:32629939] [PMC:PMC7370160]
3
Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model. Benoit Dechaumet, Damien Cleret, Marie-Thérèse Linossier, Arnaud Vanden-Bossche, Stéphanie Chanon, Etienne Lefai, Norbert Laroche, Marie-Hélène Lafage-Proust, Laurence Vico. PloS one. 2020 December;15:e0243098. [Full Text Article] [PubMed:33296408] [PMC:PMC7725345]
4
Effect of xylo-oligosaccharides on reproducibility, lipid metabolism, and adipokines of hens during the late egg-laying period. Fengyun Wen, Fengyan Wang, Pei Li, Hanyi Shi, Ning Liu. Animal bioscience. 2022 June; [Full Text Article] [PubMed:35760406]
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Proteins (19)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (1)
Recombinant (17)
NAMPT / Visfatin (19)
No (19)
6His, N-terminus (3)
DDK (Flag) (2)
GST (1)
His (5)
His, N-Terminal (3)
His,N-terminus (1)
His,N-terminus + GST (1)
His-T7, N-terminus (1)
Human (13)
Mouse (4)
Rat (2)
CHO Cells (1)
E. coli (12)
HEK 293 Cells (3)
Insect Cells (1)
Wheat Germ Extract (1)
Purified (11)
Low endotoxin level (11)
Biologically active (2)
NAMPT / Visfatin Protein - Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml Nampt (mouse) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
~52kDa (SDS-PAGE)
50 µg/$456
NAMPT / Visfatin Protein - Recombinant Visfatin By SDS-PAGE
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E. coli
His-T7, N-terminus
59.2 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
NAMPT / Visfatin Protein
Select
E. coli
His, N-Terminal
57 kDa
10 µg/$265; 100 µg/$365; 250 µg/$518; 50 µg/$315
Select
E. coli
His, N-Terminal
57.0 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
Select
Insect Cells
His,N-terminus + GST
Less than 1.0 EU/µg protein (determined by LAL method).
83.3 kDa
50 µg/$444
NAMPT / Visfatin Protein - 12.5% SDS-PAGE of human NAMPT stained with Coomassie Blue
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Wheat Germ Extract
GST
66.22 kDa
10 µg/$479; 25 µg/$670
Select
E. coli
His,N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
57 kDa
10 µg/$281; 50 µg/$389
Select
HEK 293 Cells
6His, N-terminus
57 kDa
100 µg/$298
Select
HEK 293 Cells
6His, N-terminus
57 kDa
200 µl/$283
Select
None
Biologically active
52.5 kD
25 µg/$484
NAMPT / Visfatin Protein
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$411; 500 µg/$985; 20 µg/$276
NAMPT / Visfatin Protein - Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml iNampt (human) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
~52kDa (SDS-PAGE)
50 µg/$456
Select
CHO Cells
DDK (Flag)
Less than 0.01 EU/µg protein (determined by LAL method).
~55kDa (SDS-PAGE)
10 µg/$302
Select
HEK 293 Cells
DDK (Flag)
Less than 0.1 EU/µg protein (determined by LAL method).
~60kDa (SDS-PAGE)
50 µg/$484; 10 µg/$330
NAMPT / Visfatin Protein
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
52.6 kDa
5 µg/$268; 25 µg/$349
Select
E. coli
156 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
17.27 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
NAMPT / Visfatin Protein
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$361; 500 µg/$739; 20 µg/$261
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
57kD
10 µg/$281; 50 µg/$395; 500 µg/$1,585; 1 mg/$2,157
Select
E. coli
His, N-Terminal
57.0kDa
10 µg/$291; 50 µg/$502; 200 µg/$1,002; 100 µg/$802; 1 mg/$2,144; 500 µg/$1,668; 5 mg/$3,501; 2 mg/$2,334
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).