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MRPL32

mitochondrial ribosomal protein L32

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.

Gene Name: mitochondrial ribosomal protein L32
Synonyms: MRPL32, HSPC283, L32mt, BMRP-59b, MRP-L32
Target Sequences: NM_031903 NP_114109.1 Q9BYC8

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Products
Antibodies (8)
Primary (8)
MRPL32 (8)
Human (8)
Mouse (1)
Sheep (1)
IHC (1)
WB (8)
ELISA (2)
ICC (1)
IF (5)
IP (1)
Peptide-ELISA (3)
rabbit (8)
IgG (6)
polyclonal pc (8)
Unconjugated (8)
70-150 aa, Internal (1)
Internal (1)
aa101-150 (1)
aa92-141 (1)
No (8)
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MRPL32 Rabbit anti-Human Polyclonal (70-150 aa, Internal) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
MRPL32 Antibody - Immunofluorescent analysis of MRPL32 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MRPL32 Rabbit anti-Human Polyclonal (Internal) Antibody
Sheep, Human
ICC, IF, IP, WB
Unconjugated
100 µl/$299
MRPL32 Antibody - Staining MCF-7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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MRPL32 Rabbit anti-Human Polyclonal Antibody
Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
MRPL32 Antibody - Peptide - + Immunofluorescence analysis of A549 cells, using MRPL32 antibody.
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MRPL32 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$357
MRPL32 Antibody - Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is blocked with the synthesized peptide.
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MRPL32 Rabbit anti-Human Polyclonal (aa101-150) Antibody
Human
IF, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
MRPL32 Antibody - Western blot analysis of the lysates from HeLa cells using MRPL32 antibody.
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MRPL32 Rabbit anti-Human Polyclonal (aa92-141) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
MRPL32 Antibody - Anti-MRPL32 Antibody at 1:1000 dilution + Jurkat whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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MRPL32 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$386
MRPL32 Antibody - Western blot analysis of extracts of various cell lines, using MRPL32 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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MRPL32 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
Viewing 1-8 of 8 product results


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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).