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MGMT

O-6-methylguanine-DNA methyltransferase

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene Name: O-6-methylguanine-DNA methyltransferase
Synonyms: MGMT
Target Sequences: NM_002412 NP_002403.2 P16455

Publications (7)

1
Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine. Liu L, Spiro TP, Qin X, Majka S, Haaga J, Schupp J, Willson JK, Gerson SL. Clinical cancer research : an official journal of the American Association for Cancer Research. 2001 7:2318-24. (IHC; Human) [PubMed:11489807]
2
Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection. Roth TJ, Xu Y, Luo M, Kelley MR. Cancer gene therapy. 2003 10:603-10. (WB) [PubMed:12872142]
3
O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Zuo C, Ai L, Ratliff P, Suen JY, Hanna E, Brent TP, Fan CY. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2004 13:967-75. (IHC; Human) [PubMed:15184253]
4
Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents. Cai S, Xu Y, Cooper RJ, Ferkowicz MJ, Hartwell JR, Pollok KE, Kelley MR. Cancer research. 2005 65:3319-27. (WB) [PubMed:15833865]
5
MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?. Della Puppa A, Persano L, Masi G, Rampazzo E, Sinigaglia A, Pistollato F, Denaro L, Barzon L, Pal G, Basso G, Scienza R, d'Avella D. Journal of neuro-oncology. 2012 106:33-41. [PubMed:21725802]
6
Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Molecular carcinogenesis. 2013 52:275-85. (WB; Mouse) [PubMed:22213062]
7
[Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]. Lylo VV, Matsevich LL, Kotsarenko EV, Babenko LA, Korneliuk AI, Sukhorada EM, Lukash LL. TSitologiia i genetika. 45:53-60. [PubMed:22329163]
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☰ Filters
Products
Proteins (12)
Over-Expression Lysate (2)
Recombinant (10)
MGMT (12)
No (12)
6His, N-terminus (3)
GST (1)
GST, N-terminus (1)
His (1)
His, N-Terminal (1)
His-GST (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (10)
Rat (1)
293T Cells (1)
E. coli (7)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (3)
Low endotoxin level (3)
MGMT Protein
Select
E. coli
His, N-Terminal
26.2 kDa
50 µg/$419
MGMT Protein - 12.5% SDS-PAGE of human MGMT stained with Coomassie Blue
Select
Wheat Germ Extract
GST
48.4 kDa
10 µg/$479; 25 µg/$670
MGMT Protein - Recombinant  O-6-Methylguanine DNA Methyltransferase By SDS-PAGE
Select
E. coli
His-T7
25.3 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
MGMT Protein - Recombinant O-6-Methylguanine DNA Methyltransferase By SDS-PAGE
Select
E. coli
His-GST
52.2 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
MGMT Protein - Purified recombinant protein MGMT was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
24.9 kDa
20 µg/$1,107
MGMT Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
MGMT Protein
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$411; 500 µg/$985; 20 µg/$276
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
23.8kD
10 µg/$281; 50 µg/$420; 500 µg/$1,585; 1 mg/$2,157
MGMT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
24.9 kDa
20 µg/$150
MGMT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
24.9 kDa
100 µg/$494
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
23.8 kDa
10 µg/$368; 50 µg/$504
MGMT Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).