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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KSR1

kinase suppressor of ras 1

Kinase suppressor of Ras (KSR) is involved in the regulation of the Ras activated MAPK pathway. KSR contains some kinase activity, phosphorylating Raf-1 at Thr269 in response to Ras stimulation. One study found this was necessary for the optimal activation of Raf-1 in response to EGF. Further studies popularly supported the idea that KSR acts as a scaffolding protein for the Ras stimulated MAPK pathway by supporting the formation of a large complex. Within human embryonic kidney cells KSR forms part of a multimolecular complex consisting of MEK1, MEK2, HSP90, HSP70, HSP68, p50CDC37, and 14-3-3, along with other currently unidentified proteins. The binding of protein 14-3-3 to KSR is associated with KSR phosphorylation sites Ser297 and Ser392. These sites are phosphorylated when KSR is inactive. Three other important sites on KSR are Thr260, Thr274, and Ser443, which appear to be phosphorylated by active MAPKs. Inactive ERK MAPKs may also transiently associate with the complex. These kinases will be phosphorylated by the MEK part of the KSR signaling complex.

Gene Name: kinase suppressor of ras 1
Family/Subfamily: Protein Kinase , Raf
Synonyms: KSR1, B-KSR1, KSR, RSU2, Kinase suppressor of ras, Kinase suppressor of ras 1
Target Sequences: U43586 AAC50354.1 Q8IVT5

Filtered By: None
Target
KSR1 (40)
Reactivity
Human (33)
Mouse (17)
Rat (5)
Monkey (1)
Application
IHC (13)
IHC-P (4)
WB (38)
ELISA (30)
ICC (1)
IF (3)
Peptide-ELISA (1)
Host
rabbit (33)
mouse (7)
Isotype
IgG (28)
IgG2a,k (7)
Clonality
monoclonal mc (7)
polyclonal pc (33)
Format
AP Conjugated (4)
APC Conjugated (4)
Azide-free (3)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (4)
Unconjugated (16)
Epitope
aa1-30 (8)
aa159-194 (8)
aa719-747 (8)
Arg386 (1)
Internal (1)
aa301-400 (1)
aa358-407 (1)
pSer392 (1)
pSer404 (1)
Publications
No (40)
Showing 1-25 of 40 results

KSR1 Antibody (aa358-407) LS-C117567
Rabbit Polyclonal (IgG) to Human KSR1
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KSR Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using KSR Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KSR Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using KSR Antibody. The picture on the right is blocked with the synthesized peptide.

KSR1 Antibody (Internal) LS-C358791
Rabbit Polyclonal to Human KSR1
Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of KSR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of KSR1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of KSR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of KSR1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

KSR1 Antibody (Arg386) LS-C176570
Rabbit Polyclonal to Human KSR1
Mouse, Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of KSR (R386)pAb in paraffin-embedded human brain tissue.
Western blot of KSR (R386) pAb in extracts from NIH/3T3 cells.
IHC of KSR (R386)pAb in paraffin-embedded human brain tissue.
Western blot of KSR (R386) pAb in extracts from NIH/3T3 cells.

KSR1 Antibody (aa1-30) LS-C327735
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

KSR1 Antibody (aa1-30, Biotin) LS-C219904
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

KSR1 Antibody (aa1-30, FITC) LS-C219908
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

KSR1 Antibody (aa1-30, AP) LS-C219902
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

KSR1 Antibody (aa1-30, APC) LS-C219903
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

KSR1 Antibody (aa1-30, Azide-free, HRP) LS-C219906
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

KSR1 Antibody (aa1-30, PE) LS-C219909
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

KSR1 Antibody (aa1-30) LS-C97199
Rabbit Polyclonal to Human KSR1
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-KSR1 antibody in A375 cell lysate. KSR1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-KSR1 antibody in A375 cell lysate. KSR1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

KSR1 Antibody (aa719-747) LS-C329754
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
Unconjugated
200µl  $530.00

KSR1 Antibody (aa719-747, Biotin) LS-C215551
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
Biotin Conjugated
200µl  $680.00

KSR1 Antibody (aa719-747, FITC) LS-C215552
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
FITC Conjugated
200µl  $680.00

KSR1 Antibody (aa719-747, APC) LS-C215550
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
APC Conjugated
200µl  $680.00

KSR1 Antibody (aa719-747, Azide-free, HRP) LS-C215553
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

KSR1 Antibody (aa719-747, PE) LS-C215558
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
PE Conjugated
200µl  $680.00

KSR1 Antibody (aa719-747, AP) LS-C215549
Rabbit Polyclonal (IgG) to Mouse KSR1
Mouse
ELISA WB
AP Conjugated
200µl  $680.00

KSR1 Antibody (aa301-400) LS-C309170
Mouse Monoclonal (IgG2a,k) to Human KSR1
Human
ELISA WB
Unconjugated
100µg  $560.00

KSR1 Antibody (FITC) LS-C532822
Mouse Monoclonal (IgG2a,k) to Human KSR1
Human
ELISA WB
FITC Conjugated
100µl  $710.00

KSR1 Antibody (AP) LS-C504852
Mouse Monoclonal (IgG2a,k) to Human KSR1
Human
ELISA WB
AP Conjugated
100µl  $710.00

KSR1 Antibody (APC) LS-C514563
Mouse Monoclonal (IgG2a,k) to Human KSR1
Human
ELISA WB
APC Conjugated
100µl  $710.00

KSR1 Antibody LS-C403286
Rabbit Polyclonal (IgG) to Human KSR1
Mouse, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human brain using KSR1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human brain using KSR1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human brain using KSR1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human brain using KSR1 Polyclonal Antibody at dilution of 1:30.

KSR1 Antibody LS-C406522
Rabbit Polyclonal (IgG) to Human KSR1
Mouse, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human prostate cancer using KSR1 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human prostate cancer using KSR1 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human prostate cancer using KSR1 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human prostate cancer using KSR1 Polyclonal Antibody at dilution of 1:25.

KSR1 Antibody (aa159-194) LS-C327734
Rabbit Polyclonal (IgG) to Human KSR1
Human
ELISA WB
Unconjugated
200µl 0.25mg/ml  $530.00
Showing 1-25 of 40 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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