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HSPA5 / GRP78 / BiP

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Gene Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78
Target Sequences: M19645 AAA52614.1 P11021

Publications (9)

1
NOX2 deficiency attenuates markers of adiposopathy and brain injury induced by high-fat diet. Pepping JK, Freeman LR, Gupta S, Keller JN, Bruce-Keller AJ. American journal of physiology. Endocrinology and metabolism. 2013 304:E392-404. (WB; Mouse) [PubMed:23233541] [PMC:PMC3566505]
2
Cell-extrinsic effects of tumor ER stress imprint myeloid dendritic cells and impair CD8 T cell priming. Mahadevan NR, Anufreichik V, Rodvold JJ, Chiu KT, Sepulveda H, Zanetti M. PloS one. 2012 7:e51845. [PubMed:23272178] [PMC:PMC3525659] Related Antibodies: LS-C165064.
3
Proteomic analysis of solid pseudopapillary tumor of the pancreas reveals dysfunction of the endoplasmic reticulum protein processing pathway. Zhu Y, Xu H, Chen H, Xie J, Shi M, Shen B, Deng X, Liu C, Zhan X, Peng C. Molecular & cellular proteomics : MCP. 2014 13:2593-603. (WB; Human, Rat) [Full Text Article] [PubMed:24997997] [PMC:PMC4188989]
4
Circulating GRP78 antibodies from ovarian cancer patients: a promising tool for cancer cell targeting drug delivery system?. Van Hoesen K, Meynier S, Ribaux P, Petignat P, Delie F, Cohen M. Oncotarget. 2017 November;8:107176-107187. (Human) [Full Text Article] [PubMed:29291021] [PMC:PMC5739806] Related Antibodies: LS-C165063.
5
BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway. Ledet MM, Anderson R, Harman R, Muth A, Thompson PR, Coonrod SA, Van de Walle GR. BMC cancer. 2018 April;18:412. (Human) [Full Text Article] [PubMed:29649984] [PMC:PMC5898062] Related Antibodies: LS-C312961.
6
Association of GRP78 promoter polymorphisms and serum GRP78 level with risk of asthenozoospermia. Haimei Qin, Rong Wang, Xiaoxia Pang, Yuxiao Wei, Fenglian Yang, Junli Wang. Journal of assisted reproduction and genetics. 2018 December;35:2223-2231. [Full Text Article] [PubMed:30229503] [PMC:PMC6289912]
7
Auxiliary proteins that facilitate formation of collagen-rich deposits in the posterior knee capsule in a rabbit-based joint contracture model. Andrzej Steplewski, Jolanta Fertala, Pedro K Beredjiklian , Joseph A Abboud , Mark L Y Wang , Surena Namdari , Jonathan Barlow , Michael Rivlin , William V Arnold , James Kostas, Cheryl Hou, Andrzej Fertala. The Breast : official journal of the European Society of Mastology. 2016 Mar;;34:489-501. [Full Text Article] [PubMed:26241613] Related Antibodies: LS-B4157.
8
Ultrabright plasmonic fluor nanolabel-enabled detection of a urinary ER stress biomarker in autosomal dominant tubulointerstitial kidney disease. Yeawon Kim, Zheyu Wang, Chuang Li, Kendrah Kidd, Yixuan Wang, Bryce G Johnson, Stanislav Kmoch, Jeremiah J Morrissey, Anthony J Bleyer, Jeremy S Duffield, Srikanth Singamaneni, Ying Maggie Chen. American journal of physiology. Renal physiology. 2021 July;321:F236-F244. [Full Text Article] [PubMed:34251273] [PMC:PMC8424663] Related Antibodies: LS-B2038.
9
Endoplasmic reticulum-resident chaperones modulate the inflammatory and angiogenic responses of endothelial cells. R Tsaryk, N M Bartholomä, N Simiantonaki, L Anspach, K Peters, C Heilmann, C J Kirkpatrick, F Pröls. The British journal of dermatology. 2015 August;173:416-27. [Full Text Article] [PubMed:25832777] Related Antibodies: LS-B4157.
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Proteins (17)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (3)
Recombinant (12)
HSPA5 / GRP78 / BiP (17)
No (17)
6His, N-terminus (1)
GST, N-terminus (2)
His (4)
His, N-Terminal (5)
Myc-DDK (Flag) (3)
Human (14)
Mouse (2)
293T Cells (1)
Baculovirus (1)
E. coli (7)
HEK 293 Cells (5)
Wheat Germ Extract (2)
Yeast (1)
Purified (8)
Supernatant (1)
HSPA5 / GRP78 / BiP Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
HSPA5 / GRP78 / BiP Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$570; 20 µg/$395; 1 mg/$1,973
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E. coli
His, N-Terminal
10 µg/$262; 50 µg/$339; 200 µg/$642; 1 mg/$1,623; 5 mg/$2,562; 2 mg/$1,796
HSPA5 / GRP78 / BiP Protein - Recombinant Heat Shock 70kDa Protein 5 By SDS-PAGE
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E. coli
His, N-Terminal
24.7 kDa
10 µg/$284; 50 µg/$462; 100 µg/$737; 200 µg/$922; 1 mg/$2,042; 500 µg/$1,577; 5 mg/$3,221; 2 mg/$2,231
HSPA5 / GRP78 / BiP Protein - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
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HEK 293 Cells
His, N-Terminal
~80-100 kDa
10 µg/$289; 50 µg/$360; 200 µg/$646
HSPA5 / GRP78 / BiP Protein - Purified recombinant protein HSPA5 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
72.2 kDa
20 µg/$1,107
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HEK 293 Cells
His, N-Terminal
~70 kDa
200 µl/$283
HSPA5 / GRP78 / BiP Protein - SDS-PAGE of 78kDa Grp78 (Bip) protein.
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E. coli
His
50 µg/$320; 100 µg/$416
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HEK 293 Cells
His, N-Terminal
~70 kDa
100 µg/$298
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E. coli
None
71 kDa
1000 µg/$3,417; 5 µg/$329; 20 µg/$412
HSPA5 / GRP78 / BiP Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
HSPA5 / GRP78 / BiP Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
72.2 kDa
100 µg/$494
HSPA5 / GRP78 / BiP Protein - SDS-PAGE of 78kDa native human Grp78 protein.
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E. coli
None
50 µg/$336; 100 µg/$442
HSPA5 / GRP78 / BiP Protein - 12.5% SDS-PAGE of human HSPA5 stained with Coomassie Blue
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Wheat Germ Extract
GST, N-terminus
95.7 kDa
10 µg/$479; 25 µg/$670
HSPA5 / GRP78 / BiP Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
72.2 kDa
20 µg/$150
HSPA5 / GRP78 / BiP Protein
Select
E. coli
His
100 µg/$411; 500 µg/$985; 20 µg/$276
HSPA5 / GRP78 / BiP Protein
Select
Baculovirus
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).