LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

HLA-A

Major Histocompatibility Complex, Class I, A Heavy Chain

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

Gene Name: Major Histocompatibility Complex, Class I, A Heavy Chain
Synonyms: HLA-A, Leukocyte antigen class I-A, HLAA, HLA class I histocompatibility antigen, A-1 alpha chain
Target Sequences: NM_002116 NP_002107.3 P04439

Filtered By: None
Target
HLA-A (82)
Reactivity
Human (72)
Mouse (13)
Rat (3)
Rabbit (4)
Application
IHC (10)
IHC-Fr (1)
IHC-P (7)
WB (37)
Flo (45)
Ctox (26)
ELISA (9)
Func (1)
ICC (1)
IF (17)
IP (4)
Host
rabbit (20)
mouse (58)
guinea pig (4)
Isotype
IgG (21)
IgG1 (8)
IgG1,k (3)
IgG2a (4)
IgG2a,k (1)
IgG2b (5)
IgM (33)
Clonality
monoclonal mc (57)
polyclonal pc (25)
Clone
108-2C5 (2)
2D6 (1)
7G7F9 (5)
BB7.2 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (2)
Biotin Conjugated (12)
FITC Conjugated (7)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (54)
Epitope
aa70-99 (9)
aa62-111 (4)
C-Terminus (1)
aa82-106 (1)
Publications
No (82)
Showing 1-25 of 82 results

HLA-A Antibody IHC-plus™ LS-B10898
Rabbit Polyclonal (IgG) to Human HLA-A
Human
IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-HLA-A antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE HLA-A antibody diluted at 1:1000
Anti-HLA-A antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE HLA-A antibody diluted at 1:1000

HLA-A Antibody (clone 108-2C5) LS-C390299
Mouse Monoclonal [clone 108-2C5] (IgG1,k) to Human HLA-A
Human
Flo IF
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg  $395.00

HLA-A Antibody (clone 108-2C5, Azide-free) LS-C390307
Mouse Monoclonal [clone 108-2C5] (IgG1,k) to Human HLA-A
Human
Flo IF
Unconjugated, Azide-free
100µg 1mg/ml  $415.00

HLA-A Antibody (Biotin) LS-C24460
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Biotin Conjugated
250µl  $415.00

HLA-A Antibody (aa70-99) IHC-plus™ LS-B10658
Mouse Monoclonal (IgG1) to Human HLA-A
Mouse, Human
IHC IHC-P WB
Unconjugated
200µl  $425.00
Anti-HLA-A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
HLA-A Antibody western blot of mouse Liver tissue lysates (35 ug/lane). The HLA-A antibody detected the HLA-A protein (arrow).
Anti-HLA-A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
HLA-A Antibody western blot of mouse Liver tissue lysates (35 ug/lane). The HLA-A antibody detected the HLA-A protein (arrow).

HLA-A Antibody IHC-plus™ LS-B13866
Rabbit Polyclonal (IgG) to Human HLA-A
Mouse, Human
IF IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of U2OS cell using HLA-A antibody. Blue: DAPI for nuclear staining
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of U2OS cell using HLA-A antibody. Blue: DAPI for nuclear staining

HLA-A Antibody (Biotin) LS-C24430
Mouse Monoclonal (IgG2a) to Human HLA-A
Human
Ctox Flo IF
Biotin Conjugated
250µl  $415.00

HLA-A Antibody (Biotin) LS-C24417
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Biotin Conjugated
250µl  $405.00

HLA-A Antibody (FITC) LS-C24443
Mouse Monoclonal (IgG2b) to Human HLA-A
Human
Flo IF
FITC Conjugated
250µl  $415.00

HLA-A Antibody LS-C24433
Mouse Monoclonal (IgG2a) to Human HLA-A
Human
Ctox Flo
Unconjugated
100µl  $415.00

HLA-A Antibody LS-C660292
Rabbit Polyclonal (IgG) to Human HLA-A
Human
ELISA Flo IF IHC IP WB
Unconjugated
100µg  $465.00

HLA-A Antibody LS-C482358
Rabbit Polyclonal to Human HLA-A
Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunohistochemical analysis of HLA-A staining in mouse liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of HLA-A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of HLA-A staining in mouse liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of HLA-A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

HLA-A Antibody (clone BB7.2) LS-C123772
Mouse Monoclonal [clone BB7.2] (IgG2b) to Human HLA-A
Human
Flo Func IHC IHC-Fr IP
Unconjugated
500µg  $725.00

HLA-A Antibody LS-C751995
Mouse Monoclonal (IgG2a) to Human HLA-A
Human
ELISA IHC WB
Unconjugated
100µg  $375.00

HLA-A Antibody LS-C660293
Rabbit Polyclonal (IgG) to Human HLA-A
Human
ELISA IHC WB
Unconjugated
100µg  $465.00

HLA-A Antibody LS-C24453
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Unconjugated
100µl  $385.00

HLA-A Antibody LS-C24459
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Unconjugated
100µl  $415.00

HLA-A Antibody (aa82-106) LS-C357563
Rabbit Polyclonal to Human HLA-A
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
HLA-A antibody IHC-paraffin: Human Intestinal Cancer Tissue.
HLA-A antibody IHC-paraffin: Rat Intestine Tissue.
HLA-A antibody IHC-paraffin: Human Intestinal Cancer Tissue.
HLA-A antibody IHC-paraffin: Rat Intestine Tissue.

HLA-A Antibody LS-C490054
Rabbit Polyclonal (IgG) to Human HLA-A
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µg  $365.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

HLA-A Antibody (clone 2D6) LS-C197413
Mouse Monoclonal [clone 2D6] (IgG2a,k) to Human HLA-A
Human
ELISA WB
Unconjugated
100µg  $355.00
HLA-A monoclonal antibody (M01), clone 2D6. Western Blot analysis of HLA-A expression in A-431.
Western Blot analysis of HLA-A expression in transfected 293T cell line by HLA-A monoclonal antibody (M01), clone 2D6.Lane 1: HLA-A transfected lysate (Predicted MW: 40.8 KDa).Lane 2: Non-transfected lysate.
HLA-A monoclonal antibody (M01), clone 2D6. Western Blot analysis of HLA-A expression in A-431.
Western Blot analysis of HLA-A expression in transfected 293T cell line by HLA-A monoclonal antibody (M01), clone 2D6.Lane 1: HLA-A transfected lysate (Predicted MW: 40.8 KDa).Lane 2: Non-transfected lysate.

HLA-A Antibody (Biotin) LS-C24439
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Biotin Conjugated
250µl  $415.00

HLA-A Antibody (Biotin) LS-C24456
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Biotin Conjugated
250µl  $415.00

HLA-A Antibody (Biotin) LS-C24419
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Biotin Conjugated
250µl  $415.00

HLA-A Antibody (Biotin) LS-C24462
Mouse Monoclonal (IgM) to Human HLA-A
Human
Flo IF
Biotin Conjugated
250µl  $415.00

HLA-A Antibody (FITC) LS-C24444
Mouse Monoclonal (IgG2b) to Human HLA-A
Human
Flo IF
FITC Conjugated
250µl  $560.00
Showing 1-25 of 82 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy