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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HCF1 / HCFC1

host cell factor C1 (VP16-accessory protein)

Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.

Gene Name: host cell factor C1 (VP16-accessory protein)
Synonyms: HCFC1, CFF, HFC1, Host cell factor 1, Host Cell Factor C1, HCF-1, HCF1, MRX3, VCAF, C1 factor, HCF, VP16 accessory protein
Target Sequences: NM_005334 NP_005325.2 P51610

Filtered by: None
Target
HCF1 / HCFC1 (42)
Reactivity
Human (34)
Mouse (35)
Rat (16)
Bat (4)
Bovine (12)
Chimpanzee (9)
Dog (12)
Guinea pig (8)
Hamster (8)
Horse (8)
Monkey (8)
Pig (12)
Rabbit (12)
Xenopus (4)
Zebrafish (5)
Application
IHC (8)
IHC-P (2)
WB (36)
ELISA (7)
IF (1)
IP (2)
Peptide-ELISA (1)
Host
rabbit (42)
Isotype
IgG (16)
Clonality
polyclonal pc (42)
Format
APC (2)
APC Conjugated (2)
Biotin Conjugated (6)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (6)
HRP Conjugated (6)
PE Conjugated (2)
Unconjugated (16)
Epitope
aa107-332 (8)
aa52-258 (8)
N-Terminus (5)
C-Terminus (4)
aa91-140 (4)
100-180 aa, N-terminal (1)
aa131-180 (1)
aa1700-1750 (1)
aa1985-2035 (1)
Publications
No (42)
Showing 1-25 of 42 results

IHC-plus™ HCF1 / HCFC1 Antibody (aa131-180) LS-B7675
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Mouse, Human
IHC, IHC-P, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $425.00
Anti-HCFC1 / HCF-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

HCF1 / HCFC1 Antibody LS-C499017
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Human
ELISA, IF, IHC
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human colon cancer using HCFC1 Antibody at dilution of 1:100
Immunofluorescent analysis of Hela cells using HCFC1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

HCF1 / HCFC1 Antibody (HRP) LS-C499034
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

HCF1 / HCFC1 Antibody (Biotin) LS-C499019
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

HCF1 / HCFC1 Antibody LS-C666087
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Human
ELISA, IHC, WB
Unconjugated
100µg  $355.00

HCF1 / HCFC1 Antibody LS-C809580
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

HCF1 / HCFC1 Antibody (N-Terminus) LS-C353996
Rabbit Polyclonal to Human HCF1 / HCFC1
Zebrafish, Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of HCFC1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of HCFC1 expression in HeLa (A); mouse brain (B) whole cell lysates.

HCF1 / HCFC1 Antibody (100-180 aa, N-terminal) LS-C383888
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Mouse, Human
ELISA, IHC
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

HCF1 / HCFC1 Antibody LS-C193033
Rabbit Polyclonal to Human HCF1 / HCFC1
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $385.00
Western blot of extracts of HEK-293 cell lines, using HCFC1 antibody.

HCF1 / HCFC1 Antibody LS-C331718
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human rectal cancer tissue.
Western blot analysis of extracts of various cells.

HCF1 / HCFC1 Antibody (aa1700-1750) LS-C288193
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Human
IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

HCF1 / HCFC1 Antibody (aa1985-2035) LS-C288195
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Human
IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

HCF1 / HCFC1 Antibody (aa107-332) LS-C374353
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, HRP) LS-C711207
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
HRP Conjugated
100µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $345.00; 1ml 0.5mg/ml  $695.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, Biotin) LS-C686867
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
Biotin Conjugated
100µl 0.5mg/ml  $275.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $590.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, FITC) LS-C693639
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
FITC Conjugated
100µl 0.5mg/ml  $285.00; 200µl 0.5mg/ml  $335.00; 1ml 0.5mg/ml  $650.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, APC) LS-C740415
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
APC Conjugated
100µl 0.1mg/ml  $275.00; 200µl 0.1mg/ml  $325.00; 1ml 0.1mg/ml  $605.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, Cy3) LS-C702179
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
Cy3 Conjugated
100µl 0.1mg/ml  $365.00; 200µl 0.1mg/ml  $445.00; 1ml 0.1mg/ml  $1,080.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, APC, Cy7) LS-C734229
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
APC, Cy7 Conjugated
100µl 0.1mg/ml  $385.00; 200µl 0.1mg/ml  $485.00; 1ml 0.1mg/ml  $1,215.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody (aa107-332, PE) LS-C725264
Rabbit Polyclonal to Mouse HCF1 / HCFC1
Mouse
WB
PE Conjugated
100µl 0.1mg/ml  $255.00; 200µl 0.1mg/ml  $285.00; 1ml 0.1mg/ml  $485.00
Western blot of HCF1 / HCFC1 antibody.

HCF1 / HCFC1 Antibody LS-C767108
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Mouse, Rat, Human
WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

HCF1 / HCFC1 Antibody (C-Terminus) LS-C110294
Rabbit Polyclonal to Human HCF1 / HCFC1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
HCFC1 / HCF-1 antibody Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

HCF1 / HCFC1 Antibody (N-Terminus) LS-C186676
Rabbit Polyclonal to Human HCF1 / HCFC1
Zebrafish, Pig, Rat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Western blot of Human Uterus Tumor. HCFC1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

HCF1 / HCFC1 Antibody (aa91-140) LS-C31623
Rabbit Polyclonal (IgG) to Human HCF1 / HCFC1
Xenopus, Bat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

HCF1 / HCFC1 Antibody (aa52-258) LS-C374351
Rabbit Polyclonal to Human HCF1 / HCFC1
Mouse, Human
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00
Western blot of HCF1 / HCFC1 antibody.
Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 1µg/ml Rabbit Anti-Human HCFC1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Showing 1-25 of 42 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).