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GRAP

GRB2-related adaptor protein

GRAP is a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.

Gene Name: GRB2-related adaptor protein
Synonyms: GRAP, GRB2-related adaptor protein, GRB2-related adapter protein
Target Sequences: NM_006613 NP_006604.1 Q13588

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Products
Antibodies (23)
Primary (23)
GRAP (23)
Human (22)
Mouse (13)
Rat (12)
Bat (3)
Bovine (3)
Dog (3)
Guinea pig (6)
Hamster (1)
Horse (3)
Monkey (5)
Orangutan (3)
IHC (5)
IHC-P (1)
WB (21)
ELISA (4)
ICC (2)
IF (2)
Peptide-ELISA (3)
rabbit (22)
goat (1)
IgG (10)
polyclonal pc (23)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (14)
N-Terminus (4)
aa102-151 (4)
aa143-192 (4)
10-90 aa, N-terminal (1)
aa1-50 (1)
aa155-217 (1)
aa205-217 (1)
No (23)
GRAP Antibody - 1:100 staining human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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GRAP Rabbit anti-Human Polyclonal Antibody
Mouse, Human, Monkey
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
GRAP Antibody - IHC of paraffin-embedded U373 xenograft using GRAP antibody at 1:100 dilution.
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GRAP Rabbit anti-Human Polyclonal (aa155-217) Antibody
Mouse, Rat, Human
ICC, IHC, IHC-P, WB
Unconjugated
100 µl/$450
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GRAP Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
GRAP Antibody - Western blot of Grap antibody
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GRAP Rabbit anti-Human Polyclonal (10-90 aa, N-terminal) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
GRAP Antibody - Immunofluorescence staining of GRAP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human GRAP polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Centrosome.
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GRAP Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF
Unconjugated
100 µl/$412
GRAP Antibody - Immunohistochemistry of paraffin-embedded Human gastric cancer tissue  using GRAP Polyclonal Antibody at dilution of 1:40(×200)
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GRAP Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
GRAP Antibody - Western blot analysis of extracts from COLO cells, MCF-7 cells and 293 cells, using GRAP antibody.
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GRAP Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
GRAP Antibody - Western blot analysis of lysates from 293, MCF-7, COLO cells, using GRAP Antibody. The lane on the right is blocked with the synthesized peptide.
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GRAP Rabbit anti-Human Polyclonal (aa1-50) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
GRAP Antibody - Antibody (0.3 ug/ml) staining of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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GRAP Goat anti-Human Polyclonal (aa205-217) Antibody
Mouse, Rat, Hamster, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100 µg/$503
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GRAP Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
GRAP Antibody - GRAP antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (aa143-192) Antibody
Human
WB
Unconjugated
100 µl/$424
GRAP Antibody - GRAP antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (aa143-192) (HRP) Antibody
Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Human
WB
HRP Conjugated
100 µl/$469
GRAP Antibody - GRAP antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (aa143-192) (Biotin) Antibody
Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Human
WB
Biotin Conjugated
100 µl/$469
GRAP Antibody - GRAP antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (aa143-192) (FITC) Antibody
Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Human
WB
FITC Conjugated
100 µl/$469
GRAP Antibody - Western blot of Human Fetal Brain. GRAP antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
GRAP Antibody - Western blot of Human Fetal Brain. GRAP antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$469
GRAP Antibody - Western blot of Human Fetal Brain. GRAP antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$469
GRAP Antibody - Western blot of Human Fetal Brain. GRAP antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$469
GRAP Antibody - Western blot analysis of extracts of various cell lines, using GRAP antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
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GRAP Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
GRAP Antibody - GRAP antibody Western Blot of Rat Spleen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Rat Polyclonal (aa102-151) Antibody
Rat
WB
Unconjugated
100 µl/$424
GRAP Antibody - GRAP antibody Western Blot of Rat Spleen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Rat Polyclonal (aa102-151) (HRP) Antibody
Mouse, Dog, Guinea pig, Rat, Orangutan, Human, Monkey
WB
HRP Conjugated
100 µl/$469
GRAP Antibody - GRAP antibody Western Blot of Rat Spleen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Rat Polyclonal (aa102-151) (Biotin) Antibody
Mouse, Dog, Guinea pig, Rat, Orangutan, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
GRAP Antibody - GRAP antibody Western Blot of Rat Spleen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GRAP Rabbit anti-Rat Polyclonal (aa102-151) (FITC) Antibody
Mouse, Dog, Guinea pig, Rat, Orangutan, Human, Monkey
WB
FITC Conjugated
100 µl/$469
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).