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GPNMB / Osteoactivin

glycoprotein (transmembrane) nmb

GPNMB / Osteoactivin is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: glycoprotein (transmembrane) nmb
Synonyms: GPNMB, Glycoprotein nmb-like protein, HGFIN, Glycoprotein NMB, Osteoactivin, Transmembrane glycoprotein NMB
Target Sequences: NM_002510 NP_002501.1 Q14956

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Products
Proteins (17)
Over-Expression Lysate (2)
Recombinant (15)
GPNMB / Osteoactivin (17)
SDS-PAGE (3)
No (17)
6His, C-terminus (2)
Fc, C-terminus (1)
GST (1)
GST, N-terminus (1)
His (5)
His,C-terminus (2)
Human IgG Fc, C-terminus (1)
Myc-DDK (Flag) (3)
Human (13)
Mouse (3)
293T Cells (1)
E. coli (3)
HEK 293 Cells (7)
Human Cells (3)
Mammalian Cells (1)
Wheat Germ Extract (2)
Purified (4)
Low endotoxin level (11)
Biologically active (1)
Select
Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
53.09kD
10 µg/$257; 50 µg/$355; 500 µg/$1,439; 1 mg/$2,055
GPNMB / Osteoactivin Protein
Select
E. coli
His
100 µg/$351; 500 µg/$694
GPNMB / Osteoactivin Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$461; 25 µg/$611
GPNMB / Osteoactivin Protein
Select
HEK 293 Cells
His,C-terminus
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant human GPNMB consists of 464 amino acids and predictes a molecular mass of 52.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhGPNMB is approximately 85-90 kDa due to glycosylation.
50 µg/$338; 100 µg/$431
GPNMB / Osteoactivin Protein
Select
HEK 293 Cells
Human IgG Fc, C-terminus
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant human GPNMB/Fc is a disulfide-linked homodimer. The reduced monomer comprises 694 amino acids and has a predicted molecular mass of 77.8 kDa. The apparent molecular mass of the protein is approximately 114 kDa in SDS-PAGE under reducing conditions.
50 µg/$338; 100 µg/$431
GPNMB / Osteoactivin Protein - Purified recombinant protein GPNMB was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
61.5 kDa
20 µg/$1,005
GPNMB / Osteoactivin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
63.92 kDa
20 µg/$130
GPNMB / Osteoactivin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
63.92 kDa
100 µg/$458
GPNMB / Osteoactivin Protein - 12.5% SDS-PAGE of human GPNMB stained with Coomassie Blue
Select
Wheat Germ Extract
GST
88.44 kDa
2 µg/$421
Select
Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
52.1 kDa
50 µg/$473; 100 µg/$677
GPNMB / Osteoactivin Protein
Select
HEK 293 Cells
His,C-terminus
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant mouse GPNMB comprises 490 amino acids and has a predicted molecular mass of 54.9 kDa. The apparent molecular mass of the protein is approximately 96 kDa in SDS-PAGE under reducing conditions due to glycosylation.
50 µg/$338; 100 µg/$431
GPNMB / Osteoactivin Protein - Human Osteoactivin / GPNMB, human recombinant
Select
HEK 293 Cells
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
52.9 kDa, DTT-reduced Protein migrates as 85-100 kDa due to glycosylation.
10 µg/$363
Select
HEK 293 Cells
Fc, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
77.8 kDa
50 µg/$473; 100 µg/$677
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
53.1 kDa
10 µg/$259; 50 µg/$357
Select
E. coli
99 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
11 kDa
10 mg/$4,268; 5 mg/$3,202; 1 mg/$1,868; 0.5 mg/$1,362; 200 µg/$1,023; 100 µg/$710; 50 µg/$521
Select
E. coli
164 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
18.15 kDa
10 mg/$4,268; 5 mg/$3,202; 1 mg/$1,868; 0.5 mg/$1,362; 200 µg/$1,023; 100 µg/$710; 50 µg/$521
Select
Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
54.9 kDa
50 µg/$473; 100 µg/$677
Viewing 1-17 of 17 product results


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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).