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ENO1 / Alpha Enolase

enolase 1, (alpha)

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Gene Name: enolase 1, (alpha)
Synonyms: ENO1, Alpha Enolase, Alpha enolase like 1, ENO1L1, Enolase 1, MBP-1, MBPB1, MPB1, MYC promoter-binding protein 1, NNE, Phosphopyruvate hydratase, Alpha-enolase, Tau-crystallin, MBP1, MPB-1, C-myc promoter-binding protein, Enolase 1, (alpha), Enolase-alpha, Non-neural enolase, Plasminogen-binding protein, PPH
Target Sequences: NM_001428 NP_001419.1 P06733

Publications (1)

1
Characterization of brain tumor initiating cells isolated from an animal model of CNS primitive neuroectodermal tumors. Malchenko S, Sredni ST, Boyineni J, Bi Y, Margaryan NV, Guda MR, Kostenko Y, Tomita T, Davuluri RV, Velpula K, Hendrix MJC, Soares MB. Oncotarget. 2018 February;9:13733-13747. (Human) [Full Text Article] [PubMed:29568390] [PMC:PMC5862611] Related Antibodies: LS-B10960.

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Products
Proteins (20)
Over-Expression Lysate (2)
Recombinant (18)
ENO1 / Alpha Enolase (20)
No (20)
10His, N-terminus (1)
6His, C-terminus (2)
6His, N-terminus (1)
GST (3)
GST, N-terminus (1)
His (5)
His-T7 (2)
MBP, N-terminus + 6His, C-terminus (1)
Myc-DDK (Flag) (3)
Aspergillus fumigatus (1)
Bovine (1)
Human (14)
Mouse (3)
293T Cells (1)
Baculovirus (1)
E. coli (12)
HEK 293 Cells (2)
Wheat Germ Extract (3)
Yeast (1)
Purified (6)
Low endotoxin level (2)
Biologically active (1)
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E. coli
GST
73.7 kD
100 µg/$420; 20 µg/$323; 1 mg/$1,355
ENO1 / Alpha Enolase Protein
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E. coli
None
Biologically active
100 µg/$342; 500 µg/$641; 20 µg/$254
ENO1 / Alpha Enolase Protein - Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of Recombinant Neosartorya fumigata Enolase(enoA) could indicate that this peptide derived from E.coli-expressed Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) enoA.
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E. coli
6His, N-terminus
51.2 kDa
1 mg/$1,679; 20 µg/$405; 100 µg/$598
ENO1 / Alpha Enolase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Baculovirus
MBP, N-terminus + 6His, C-terminus
91.0 kDa
100 µg/$1,067; 20 µg/$447; 1 mg/$1,988
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E. coli
6His, C-terminus
Less than 0.1 ng/mL (Less than 1 EU/µg) (determined by LAL method).
10 µg/$321; 50 µg/$534; 500 µg/$1,680; 1 mg/$2,353
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
48 kDa
10 µg/$344; 50 µg/$643
Select
Yeast
His
51 kD
1 mg/$1,679; 100 µg/$500; 20 µg/$364
ENO1 / Alpha Enolase Protein - 12.5% SDS-PAGE of human ENO1 stained with Coomassie Blue
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Wheat Germ Extract
GST
73.48 kDa
10 µg/$479; 25 µg/$670
ENO1 / Alpha Enolase Protein - 12.5% SDS-PAGE of human ENO1 stained with Coomassie Blue
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Wheat Germ Extract
GST
73.48 kDa
10 µg/$479; 25 µg/$670
ENO1 / Alpha Enolase Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
ENO1 / Alpha Enolase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
10His, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
ENO1 / Alpha Enolase Protein - Purified recombinant protein ENO1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
47 kDa
20 µg/$1,107
ENO1 / Alpha Enolase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
47 kDa
20 µg/$150
ENO1 / Alpha Enolase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
47 kDa
100 µg/$494
Select
E. coli
His
49.3 kDa
100 µg/$502
ENO1 / Alpha Enolase Protein - Recombinant Enolase, Non Neuronal By SDS-PAGE
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E. coli
His
48.9 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
ENO1 / Alpha Enolase Protein - Recombinant Enolase, Non Neuronal By SDS-PAGE
Select
E. coli
His-T7
51.0 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
ENO1 / Alpha Enolase Protein - Recombinant Enolase, Non Neuronal By SDS-PAGE
Select
E. coli
His-T7
21.3 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
ENO1 / Alpha Enolase Protein - Recombinant Enolase, Non Neuronal By SDS-PAGE
Select
E. coli
His
11.3 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
ENO1 / Alpha Enolase Protein - Recombinant Enolase, Non Neuronal By SDS-PAGE
Select
E. coli
His
49.5 kDa
10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).