Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
deiodinase, iodothyronine, type III
DIO3, 5DIII, D3, DIOIII, ITDI3, TXDI3, Type-III 5 deiodinase, Type-III 5-deiodinase, Type 3 DI
Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cells. Freitas BC, Gereben B, Castillo M, Kall I, Zeld A, Egri P, Liposits Z, Zavacki AM, Maciel RM, Jo S, Singru P, Sanchez E, Lechan RM, Bianco AC. The Journal of clinical investigation. 2010 120:2206-17.
Contributions of HIV infection in the hypothalamus and substance abuse/use to HPT dysregulation. Langford D, Baron D, Joy J, Del Valle L, Shack J. Psychoneuroendocrinology. 2011 36:710-9. (ICC, IHC, WB; Human)
A novel pathway regulates thyroid hormone availability in rat and human hypothalamic neurosecretory neurons. Kall I, Mohcsik P, Vida B, Zeld A, Bardczi Z, Zavacki AM, Farkas E, Kdr A, Hrabovszky E, Arrojo E Drigo R, Dong L, Barna L, Palkovits M, Borsay BA, Herczeg L, Lechan RM, Bianco AC, Liposits Z, Fekete C, Gereben B. PloS one. 2012 7:e37860. (IHC, WB; Human, Rat)
Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism. Jo S, Kall I, Bardczi Z, Arrojo e Drigo R, Zeld A, Liposits Z, Oliva A, Lemmon VP, Bixby JL, Gereben B, Bianco AC. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:8491-500. (IHC, WB; Human, Rat, Hamster)