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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DIO2

deiodinase, iodothyronine, type II

Type II iodothyronine deiodinase activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3&#039,5-triiodothyronine (T3). Thyroid hormone appears to have important regulatory effects in some mammalian tissues, such as the developing brain, the anterior pituitary gland, and brown adipose tissue. A relatively high proportion of the receptor-bound triiodothyronine is found within the tissue itself rather than in plasma. The expression in these tissues of type II iodothyronine deiodinase, suggests that deiodinase II is responsible for this &#039local&#039 production of T3 and is thus important in influencing thyroid hormone action in these tissues. In addition, DIO2 activity is markedly elevated in the hypothyroid state and appears to be responsible for catalyzing the production of a large proportion of the circulating T3 under such conditions. Dioiodinase 2 may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease, toxic adenomas, and, perhaps, iodine deficiency. Alternative splice variants are formed due to the presence or absence of an unspliced intron in the 5&#039 untranslated region.

Gene Name: deiodinase, iodothyronine, type II
Synonyms: DIO2, 5DII, D2, DIOII, ITDI2, Type 2 DI, TXDI2, Type-II 5-deiodinase, SelY, Type-II 5deiodinase, Thyroxine deiodinase, type II
Target Sequences: NM_013989 NP_054644.1 Q92813

Filtered By: None
Target
DIO2 (28)
Reactivity
Human (23)
Mouse (15)
Rat (12)
Pig (1)
Application
IHC (4)
IHC-P (2)
WB (22)
Flo (2)
ELISA (10)
ICC (2)
IF (2)
Peptide-ELISA (1)
Host
rabbit (27)
goat (1)
Isotype
IgG (26)
Clonality
polyclonal pc (28)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (8)
Epitope
aa30-259 (13)
aa165-191 (8)
aa217-228 (1)
Publications
No (28)
Showing 1-25 of 28 results

DIO2 Antibody (aa165-191) LS-C167417
Rabbit Polyclonal to Human DIO2
Mouse, Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
DIO2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody for immunohistochemistry.
Western blot of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
DIO2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody for immunohistochemistry.
Western blot of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

DIO2 Antibody (aa217-228) LS-C61730
Goat Polyclonal to Human DIO2
Mouse, Pig, Human
Flo IF IHC Peptide-ELISA
Unconjugated
100µg  $405.00
DIO2 antibody (2 ug/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
DIO2 antibody (2 ug/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.

DIO2 Antibody LS-C671307
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA IF IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .

DIO2 Antibody (HRP) LS-C671305
Rabbit Polyclonal (IgG) to Human DIO2
Human
HRP Conjugated
50µg  $285.00; 100µg  $385.00

DIO2 Antibody (Biotin) LS-C671309
Rabbit Polyclonal (IgG) to Human DIO2
Human
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

DIO2 Antibody (FITC) LS-C671308
Rabbit Polyclonal (IgG) to Human DIO2
Human
FITC Conjugated
50µg  $285.00; 100µg  $385.00

DIO2 Antibody LS-C403113
Rabbit Polyclonal (IgG) to Human DIO2
Mouse, Rat, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human cervical cancer using DIO2 Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of Human cervical cancer using DIO2 Polyclonal Antibody at dilution of 1:50.

DIO2 Antibody (aa30-259) LS-C314935
Rabbit Polyclonal (IgG) to Mouse DIO2
Mouse
WB
Unconjugated
100µl 0.5mg/ml  $355.00
Western blot of recombinant DIO2.
Western blot of recombinant DIO2.

DIO2 Antibody (aa30-259, Biotin) LS-C422812
Rabbit Polyclonal (IgG) to Mouse DIO2
Mouse
ICC WB
Biotin Conjugated
100µl 0.5mg/ml  $375.00

DIO2 Antibody (aa30-259, FITC) LS-C422777
Rabbit Polyclonal (IgG) to Mouse DIO2
Mouse
ICC WB
FITC Conjugated
100µl 0.5mg/ml  $395.00

DIO2 Antibody (aa165-191) LS-C327196
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
Unconjugated
200µl 0.25mg/ml  $530.00

DIO2 Antibody (aa165-191, Biotin) LS-C276769
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

DIO2 Antibody (aa165-191, FITC) LS-C276771
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
FITC Conjugated
200µl  $680.00

DIO2 Antibody (aa165-191, Azide-free, HRP) LS-C276772
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

DIO2 Antibody (aa165-191, APC) LS-C276770
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
APC Conjugated
200µl  $680.00

DIO2 Antibody (aa165-191, PE) LS-C276773
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
PE Conjugated
200µl  $680.00

DIO2 Antibody (aa165-191, AP) LS-C276768
Rabbit Polyclonal (IgG) to Human DIO2
Human
ELISA WB
AP Conjugated
200µl  $680.00

DIO2 Antibody LS-C406358
Rabbit Polyclonal (IgG) to Human DIO2
Mouse, Rat, Human
ELISA WB
Unconjugated
20µl  $205.00; 60µl 0.7mg/ml  $235.00; 120µl  $295.00; 200µl 0.7mg/ml  $395.00
Western blot analysis of Mouse brain tissue, using DIO2 Polyclonal Antibody at dilution of 1:200.
Western blot analysis of Mouse brain tissue, using DIO2 Polyclonal Antibody at dilution of 1:200.

DIO2 Antibody (aa30-259) LS-C293664
Rabbit Polyclonal (IgG) to Rat DIO2
Mouse, Rat, Human
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $575.00; 10ml 0.5mg/ml  $2,280.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DIO2 Antibody (aa30-259, HRP) LS-C712029
Rabbit Polyclonal (IgG) to Rat DIO2
Mouse, Rat, Human
WB
HRP Conjugated
20µl  $215.00; 100µl  $295.00; 200µl  $355.00; 1ml  $740.00; 10ml  $2,955.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DIO2 Antibody (aa30-259, Biotin) LS-C300434
Rabbit Polyclonal (IgG) to Rat DIO2
Rat
WB
Biotin Conjugated
100µl 0.5mg/ml  $385.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DIO2 Antibody (aa30-259, Biotin) LS-C687550
Rabbit Polyclonal (IgG) to Rat DIO2
Mouse, Rat, Human
WB
Biotin Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $620.00; 10ml  $2,505.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DIO2 Antibody (aa30-259, FITC) LS-C303764
Rabbit Polyclonal (IgG) to Rat DIO2
Rat
WB
FITC Conjugated
100µl 0.5mg/ml  $415.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DIO2 Antibody (aa30-259, Cy3) LS-C703004
Rabbit Polyclonal (IgG) to Rat DIO2
Mouse, Rat, Human
WB
Cy3 Conjugated
20µl  $255.00; 100µl  $375.00; 200µl  $465.00; 1ml  $1,140.00; 10ml  $4,560.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DIO2 Antibody (aa30-259, APC, Cy7) LS-C735036
Rabbit Polyclonal (IgG) to Rat DIO2
Mouse, Rat, Human
WB
APC, Cy7 Conjugated
20µl  $295.00; 100µl  $475.00; 200µl  $680.00; 1ml  $1,710.00; 10ml  $6,840.00
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant DIO2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 28 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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