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DHFR

dihydrofolate reductase

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Gene Name: dihydrofolate reductase
Synonyms: DHFR, DHFRP1, Dihydrofolate reductase, DYR
Target Sequences: NM_000791 NP_000782.1 P00374

Publications (1)

1
Oxidative stress inhibits distant metastasis by human melanoma cells. Piskounova E, Agathocleous M, Murphy MM, Hu Z, Huddlestun SE, Zhao Z, Leitch AM, Johnson TM, DeBerardinis RJ, Morrison SJ. Nature. 2015 527:186-91. (IHC, WB; Human) [Full Text Article] [PubMed:26466563] [PMC:PMC4644103]

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Products
Antibodies (27)
Primary (27)
DHFR (27)
Human (27)
Mouse (10)
Rat (8)
Dog (2)
Monkey (3)
IHC (14)
IHC-P (7)
WB (22)
Flo (1)
ELISA (9)
ICC (1)
IF (6)
IP (1)
Peptide-ELISA (1)
rabbit (21)
mouse (6)
Product Group
IHCPlus (2)
IgG (15)
IgG1 (2)
IgG1,k (1)
IgG2a,k (1)
IgG2b (2)
monoclonal mc (6)
polyclonal pc (21)
2B10 (1)
AT5B2 (1)
OTI3A11 (2)
OTI6G7 (2)
APC Conjugated (1)
Biotin Conjugated (2)
Carrier-free (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (19)
aa2-187 (5)
aa1-187 (1)
aa135-164 (1)
aa16-30 (1)
No (27)
DHFR Antibody - IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
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DHFR Mouse anti-Human Monoclonal (OTI6G7) Antibody
Dog, Human, Monkey
IF, IHC, IHC-P, WB
Unconjugated
100 µl/$434
DHFR Antibody - IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
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DHFR Mouse anti-Human Monoclonal (Carrier-free) (OTI6G7) Antibody
Dog, Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100 µg/$511
DHFR Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
DHFR Antibody - Immunofluorescence of monoclonal antibody to DHFR on HeLa cell. [antibody concentration 10 ug/ml]
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DHFR Mouse anti-Human Monoclonal (2B10) Antibody
Rat, Human
ELISA, IF, WB
Unconjugated
100 µg/$423
DHFR Antibody - DHFR Antibody IHC of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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DHFR Rabbit anti-Mouse Polyclonal (aa135-164) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
DHFR Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
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DHFR Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100 µg/$331
DHFR Antibody - 1:100 staining human brain carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human, Monkey
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
DHFR Antibody - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate
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DHFR Mouse anti-Human Monoclonal (AT5B2) Antibody
Human
ELISA, Flo, ICC, WB
Unconjugated
50 µl/$303; 100 µl/$365
DHFR Antibody - IHC of paraffin-embedded breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
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DHFR Mouse anti-Human Monoclonal (OTI3A11) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$434
DHFR Antibody - IHC of paraffin-embedded breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
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DHFR Mouse anti-Human Monoclonal (Carrier-free) (OTI3A11) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
DHFR Antibody - DHFR antibody IHC-paraffin: Mouse Brain Tissue.
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DHFR Rabbit anti-Human Polyclonal (aa2-187) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
DHFR Antibody - Immunohistochemical of paraffin-embedded H. liver section using DHFR Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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DHFR Rabbit anti-Human Polyclonal (aa16-30) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
DHFR Antibody - Immunohistochemistry of paraffin-embedded Human tonsil tissue  using DHFR Polyclonal Antibody at dilution of 1:50(×200)
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
DHFR Antibody - Immunohistochemistry of paraffin-embedded Human tonsil tissue  using DHFR Polyclonal Antibody at dilution of 1:50(×200)
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
DHFR Antibody - Western blot of extracts of various cell lines, using DHFR antibody.
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
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DHFR Rabbit anti-Human Polyclonal (aa1-187) Antibody
Human
WB
Unconjugated
100 µg/$749
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DHFR Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$937
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DHFR Rabbit anti-Human Polyclonal (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$937
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DHFR Rabbit anti-Human Polyclonal (PE) Antibody
Human
WB
PE Conjugated
100 µl/$937
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DHFR Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
DHFR Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using DHFR Antibody at dilution of 1:100
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DHFR Rabbit anti-Human Polyclonal (aa2-187) Antibody
Human
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
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DHFR Rabbit anti-Human Polyclonal (aa2-187) (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
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DHFR Rabbit anti-Human Polyclonal (aa2-187) (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
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DHFR Rabbit anti-Human Polyclonal (aa2-187) (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).