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CRYBB1

crystallin, beta B1

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3.

Gene Name: crystallin, beta B1
Synonyms: CRYBB1, Beta-crystallin B1, CATCN3, Beta-B1 crystallin, Crystallin, beta B1, Eye lens structural protein
Target Sequences: NM_001887 NP_001878.1 P53674

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Products
Proteins (8)
Over-Expression Lysate (2)
Recombinant (6)
CRYBB1 (8)
No (8)
GST (1)
GST, N-terminus (1)
His (2)
His-GST (1)
Myc-DDK (Flag) (3)
Human (5)
Mouse (1)
Rat (1)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (2)
CRYBB1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
27.8 kDa
100 µg/$494
CRYBB1 Protein - Recombinant  Crystallin Beta B1 By SDS-PAGE
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E. coli
His-GST
49.9 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
CRYBB1 Protein - 12.5% SDS-PAGE of human CRYBB1 stained with Coomassie Blue
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Wheat Germ Extract
GST
53.46 kDa
2 µg/$439
CRYBB1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
27.8 kDa
20 µg/$150
CRYBB1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
CRYBB1 Protein - Purified recombinant protein CRYBB1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
27.8 kDa
20 µg/$1,107
CRYBB1 Protein
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E. coli
His
100 µg/$342; 500 µg/$641; 20 µg/$254
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E. coli
His
21.3 kDa
10 µg/$265; 50 µg/$347; 100 µg/$545; 200 µg/$682; 1 mg/$1,690; 500 µg/$1,270; 5 mg/$2,803
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).