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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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COL4A5 / Collagen IV Alpha5

collagen, type IV, alpha 5

COL4A5 / Collagen IV Alpha5 is one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene.

Gene Name: collagen, type IV, alpha 5
Family/Subfamily: Collagen , not assigned-Collagen
Synonyms: COL4A5, Alport syndrome, ASLN, ATS, CA54, Collagen, type IV, alpha 5, DA149D17.3, Collagen alpha 5(iv), Collagen alpha-5(IV) chain, DA24A23.1
Target Sequences: NM_000495 NP_000486.1 P29400

Filtered By: None
Target
COL4A5 / Collagen IV Alpha5 (30)
Reactivity
Human (30)
Mouse (11)
Pig (8)
Application
IHC (14)
IHC-P (3)
WB (28)
ELISA (9)
ICC (1)
IF (3)
Peptide-ELISA (1)
Host
rabbit (22)
mouse (8)
Isotype
IgG (27)
Clonality
monoclonal mc (8)
polyclonal pc (22)
Format
AP Conjugated (1)
APC (2)
APC Conjugated (3)
Azide-free (1)
Biotin Conjugated (3)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (4)
HRP Conjugated (3)
PE Conjugated (3)
Unconjugated (9)
Epitope
aa1461-1685 (17)
aa230-259 (7)
C-Terminus (2)
40-120 aa, N-terminal (1)
N-Terminus (1)
aa21-70 (1)
Publications
No (30)
Showing 1-25 of 30 results

COL4A5 / Collagen IV Alpha5 Antibody IHC-plus™ LS-B15572
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Breast, Fibrous Tissue: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts of various cells.
Human Breast, Fibrous Tissue: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts of various cells.

COL4A5 / Collagen IV Alpha5 Antibody (aa21-70) LS-C119458
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Human
IF IHC IHC-P Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.

COL4A5 / Collagen IV Alpha5 Antibody (C-Terminus) LS-C153417
Rabbit Polyclonal to Human COL4A5 / Collagen IV Alpha5
Human
ELISA IHC WB
Unconjugated
100µg 100µl 1mg/ml  $345.00

COL4A5 / Collagen IV Alpha5 Antibody (N-Terminus) LS-C353940
Rabbit Polyclonal to Human COL4A5 / Collagen IV Alpha5
Mouse, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Collagen 4 alpha 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Collagen 4 alpha 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

COL4A5 / Collagen IV Alpha5 Antibody (40-120 aa, N-terminal) LS-C382814
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Human
ELISA IF IHC
Unconjugated
100µl 1mg/ml  $265.00

COL4A5 / Collagen IV Alpha5 Antibody (C-Terminus) LS-C145053
Rabbit Polyclonal to Human COL4A5 / Collagen IV Alpha5
Human
IHC WB
Unconjugated
100µl  $285.00

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685) LS-C293435
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, Biotin) LS-C300459
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
Biotin Conjugated
100µl 0.5mg/ml  $425.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259) LS-C329407
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
Unconjugated
200µl  $530.00

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259, Biotin) LS-C208518
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259, FITC) LS-C208519
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
FITC Conjugated
200µl  $680.00

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259, AP) LS-C208515
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
AP Conjugated
200µl  $680.00

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259, APC) LS-C208517
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
APC Conjugated
200µl  $680.00

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259, Azide-free, HRP) LS-C208520
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

COL4A5 / Collagen IV Alpha5 Antibody (aa230-259, PE) LS-C208521
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
ELISA WB
PE Conjugated
200µl  $680.00

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, FITC) LS-C693565
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
FITC Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $635.00; 10ml  $2,520.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, PE) LS-C725181
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
PE Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $635.00; 10ml  $2,520.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, APC, Cy7) LS-C734150
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
APC, Cy7 Conjugated
20µl  $285.00; 100µl  $455.00; 200µl  $635.00; 1ml  $1,575.00; 10ml  $6,300.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, APC) LS-C717622
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
APC Conjugated
20µl  $225.00; 100µl  $305.00; 200µl  $375.00; 1ml  $785.00; 10ml  $4,050.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, HRP) LS-C711119
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
HRP Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $345.00; 1ml  $680.00; 10ml  $2,730.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, Cy3) LS-C702095
Rabbit Polyclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Mouse, Pig, Human
IHC WB
Cy3 Conjugated
20µl  $245.00; 100µl  $355.00; 200µl  $445.00; 1ml  $1,050.00; 10ml  $4,200.00
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant COL4A5 / Collagen IV.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685) LS-C314274
Mouse Monoclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
WB
Unconjugated
20µl 1mg/ml  $205.00; 100µl 1mg/ml  $255.00; 200µl 1mg/ml  $305.00; 1ml 1mg/ml  $530.00; 10ml 1mg/ml  $2,100.00
Western blot of recombinant COL4A5 / Collagen IV.
Western blot of recombinant COL4A5 / Collagen IV.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, Cy3) LS-C702099
Mouse Monoclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
WB
Cy3 Conjugated
20µl  $245.00; 100µl  $355.00; 200µl  $445.00; 1ml  $1,050.00; 10ml  $4,215.00
Western blot of recombinant COL4A5 / Collagen IV.
Western blot of recombinant COL4A5 / Collagen IV.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, HRP) LS-C711123
Mouse Monoclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
WB
HRP Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $345.00; 1ml  $680.00; 10ml  $2,745.00
Western blot of recombinant COL4A5 / Collagen IV.
Western blot of recombinant COL4A5 / Collagen IV.

COL4A5 / Collagen IV Alpha5 Antibody (aa1461-1685, PE) LS-C725183
Mouse Monoclonal (IgG) to Human COL4A5 / Collagen IV Alpha5
Human
WB
PE Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $635.00; 10ml  $2,520.00
Western blot of recombinant COL4A5 / Collagen IV.
Western blot of recombinant COL4A5 / Collagen IV.
Showing 1-25 of 30 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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