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CA1 / Carbonic Anhydrase I

carbonic anhydrase I

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants.

Gene Name: carbonic anhydrase I
Synonyms: CA1, CAB, Carbonic anhydrase 1, CAR1, Carbonate dehydratase I, Carbonic dehydratase, CA-I, Carbonic anhydrase B, Carbonic anhydrase I
Target Sequences: NM_001738 NP_001729.1 P00915

Publications (1)

1
Identification of Candidate Biomarkers in Malignant Ascites from Patients with Hepatocellular Carcinoma by iTRAQ-Based Quantitative Proteomic Analysis. Zhang J, Liang R, Wei J, Ye J, He Q, ChunlingYuan, Ye J, Li Y, Liu Z, Lin Y. BioMed research international. 2018 September;2018:5484976. [Full Text Article] [PubMed:30345303] [PMC:PMC6174818]

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Proteins (24)
Over-Expression Lysate (10)
Recombinant (14)
CA1 / Carbonic Anhydrase I (24)
No (24)
6His, C-terminus (3)
6His, N-terminus (2)
6His-SUMO, N-terminus (1)
GST (1)
GST, N-terminus (1)
His (4)
His-SUMO (1)
Myc-DDK (Flag) (11)
Human (15)
Mouse (1)
Rat (3)
293T Cells (5)
E. coli (9)
HEK 293 Cells (6)
Wheat Germ Extract (2)
Yeast (2)
Purified (3)
Low endotoxin level (3)
CA1 / Carbonic Anhydrase I Protein - Recombinant Carbonic Anhydrase I By SDS-PAGE
Select
E. coli
6His, N-terminus
24.1 kDa
10 µg/$283; 50 µg/$452; 100 µg/$725; 200 µg/$905; 1 mg/$2,020; 500 µg/$1,558; 5 mg/$3,165; 2 mg/$2,209
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HEK 293 Cells
Myc-DDK (Flag)
28.7 kDa
100 µg/$494
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
28.7 kDa
100 µg/$494
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
28.7 kDa
100 µg/$494
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
28.7 kDa
100 µg/$494
CA1 / Carbonic Anhydrase I Protein - Purified recombinant protein CA1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
28.7 kDa
20 µg/$1,107
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E. coli
His-SUMO
44.7 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
Select
Yeast
His
30.7 kD
100 µg/$446; 20 µg/$336; 1 mg/$1,460
CA1 / Carbonic Anhydrase I Protein
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Yeast
His
30.2 kD
1 mg/$1,683; 100 µg/$499; 20 µg/$364
CA1 / Carbonic Anhydrase I Protein - 12.5% SDS-PAGE of human CA1 stained with Coomassie Blue
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Wheat Germ Extract
GST
54.45 kDa
10 µg/$479; 25 µg/$670
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293T Cells
Myc-DDK (Flag)
28.7 kDa
20 µg/$150
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
28.7 kDa
20 µg/$150
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
28.7 kDa
20 µg/$150
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
28.7 kDa
20 µg/$150
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
28.7 kDa
20 µg/$150
CA1 / Carbonic Anhydrase I Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
CA1 / Carbonic Anhydrase I Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
CA1 / Carbonic Anhydrase I Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
CA1 / Carbonic Anhydrase I Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
28.7 kDa
100 µg/$494
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
29.9 kDa
10 µg/$343; 50 µg/$429
CA1 / Carbonic Anhydrase I Protein
Select
E. coli
His
100 µg/$342; 500 µg/$641; 20 µg/$254
CA1 / Carbonic Anhydrase I Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
29.93 kDa
50 µg/$385; 10 µg/$269; 1 mg/$2,837; 500 µg/$1,987
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
29.93kD
10 µg/$255; 50 µg/$343; 500 µg/$1,585; 1 mg/$2,157
CA1 / Carbonic Anhydrase I Protein - Recombinant Carbonic Anhydrase I By SDS-PAGE
Select
E. coli
His
30.0 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).