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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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BRF1

BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

Gene Name: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Synonyms: BRF1, B-related factor 1, BRF, B - related factor 1, HBRF, GTF3B, HTFIIIB90, TAF3C, TAFIII90, TFIIIB90, TAF3B2, BRF-1, TF3B90
Target Sequences: NM_001519 NP_001510.2 Q92994

Viewing 1-25 of 41 items
BRF1 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl   0.2mg/ml   $425.00
BRF1 Antibody - Immunohistochemical analysis of BRF1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human BRF1
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
BRF1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
BRF1 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using TF3B antibody.
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Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µg   $305.00
BRF1 Antibody - Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.
Select
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
BRF1 Antibody - Western blot of TFIIIB90-1 antibody
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Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
BRF1 Antibody - Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRF1 mAb.
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Mouse Monoclonal [clone BRF1G2A8] (IgG1) to Human BRF1
Human
DB, ICC, IF, WB
Unconjugated
100µg   100µg/ml   $415.00
BRF1 Antibody - IHC of TFIIIB90-1 (E257) pAb in paraffin-embedded human brain tissue.
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Rabbit Polyclonal to Human BRF1
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl   $345.00
BRF1 Antibody - Western blot of immunized recombinant protein using BRF1 antibody.
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Mouse Monoclonal [clone BRF1G2A8] (IgG1) to Human BRF1
Human
DB, ICC, WB
Unconjugated
100µg   100µg/ml   $530.00
BRF1 Antibody - Immunofluorescence staining of BRF1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BRF1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ICC, IF
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
BRF1 Antibody - Western blot analysis of the lysates from HepG2 cells using BRF1 antibody.
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Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
BRF1 Antibody - Detection of Human BRF1 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRF1 used at a dilution of 1:200 (1 ug/ml).
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Rabbit Polyclonal (IgG) to Human BRF1
Human
IHC, IP, WB
Unconjugated
100µl   0.2mg/ml   $405.00
BRF1 Antibody - Detection limit for recombinant GST tagged BRF1 is 3 ng/ml as a capture antibody.
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Mouse Monoclonal [clone 2E9] (IgG1,k) to Human BRF1
Human
ELISA, WB
Unconjugated
50µg   $365.00
Select
Rabbit Polyclonal to Human BRF1
Human
ELISA, WB
Unconjugated
100µg   $275.00
Select
Rabbit Polyclonal to Human BRF1
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg   $315.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
APC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Mouse Polyclonal (IgG) to Human BRF1
Human
IF, WB
Unconjugated
50µg   $560.00
BRF1 Antibody - NIH 3T3 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Mouse BRF1
Mouse, Bovine, Rat
WB
Unconjugated
100µl   0.5mg/ml   $335.00
Select
Mouse Monoclonal (IgG1) to Human BRF1
Human
WB
Unconjugated
100µg   $590.00

Viewing 1-25 of 41 items


If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).