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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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BRD4

bromodomain containing 4

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

Gene Name: bromodomain containing 4
Family/Subfamily: Protein Kinase , BRD
Synonyms: BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI
Target Sequences: NM_014299 NP_055114.1 O60885

Publications (2)

1
The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Dai J, Sultan S, Taylor SS, Higgins JM. Genes & development. 2005 19:472-88. [PubMed:15681610] [PMC:PMC548948] Related Antibodies: LS-C159181.
2
Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers. Kurg R, Tekkel H, Abroi A, Ustav M. Journal of virology. 2006 80:11218-25. [PubMed:16943289] [PMC:PMC1642174] Related Antibodies: LS-C159181.

Viewing 1-25 of 52 items
BRD4 Antibody - Anti-BRD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Human BRD4
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Bat, Human
IHC, IHC-P, WB
Unconjugated
100µl   $485.00
BRD4 Antibody - Anti-BRD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human BRD4
Rabbit, Mouse, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human
IHC, IHC-P, WB
HRP Conjugated
100µl   0.65mg/ml   $405.00
BRD4 Antibody - Anti-BRD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human BRD4
Rabbit, Mouse, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human
IHC, IHC-P, WB
Biotin Conjugated
100µl   1mg/ml   $405.00
BRD4 Antibody - Anti-BRD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human BRD4
Rabbit, Mouse, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human
IHC, IHC-P, WB
FITC Conjugated
100µl   1mg/ml   $405.00
BRD4 Antibody - Western blot blot of extracts of various cell lines, using BRD4 antibody.
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Rabbit Polyclonal (IgG) to Human BRD4
Mouse, Human
WB
Unconjugated
50µl   $315.00; 100µl   $425.00; 200µl   $650.00
BRD4 Antibody - Western blot ofBRD4 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using antibody at0.5 ug/ml.
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Rabbit Polyclonal to Human BRD4
Mouse, Rat, Human
WB
Unconjugated
100µg   0.5mg/ml   $335.00
BRD4 Antibody - BRD4 Antibody western blot of T47D cell line lysates (35 ug/lane). The BRD4 antibody detected the BRD4 protein (arrow).
Select
(2) Publication(s)
Rabbit Polyclonal to Human BRD4
Human
WB
Unconjugated
400µl   $325.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC, WB
APC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC, WB
PE Conjugated
200µl   $740.00
BRD4 Antibody - Detection of Human BRD4 by Immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1000 (1 ug/ml).
Select
Rabbit Polyclonal (IgG) to Human BRD4
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
100µl   1mg/ml   $665.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, IHC
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, ICC, IF, IHC
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, ICC, IF, IHC
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, ICC, IF, IHC
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, ICC, IF, IHC
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, ICC, IF, IHC
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human BRD4
Human
ELISA, ICC, IF, IHC
APC Conjugated
200µl   $740.00
BRD4 Antibody
Select
Rabbit Polyclonal (IgG) to Human BRD4
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
BRD4 Antibody - Western blot of BRD4 antibody LS-X234994.
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Rabbit Polyclonal (IgG) to Human BRD4
Mouse, Rat, Human
WB
Unconjugated
30µg   $245.00; 100µg   $405.00
BRD4 Antibody -  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Rat BRD4
Rabbit, Mouse, Guinea pig, Rat, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
BRD4 Antibody - Detection of Human BRD4 by Immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1000 (1 ug/ml).
Select
Rabbit Polyclonal (IgG) to Human BRD4
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl   1mg/ml   $415.00

Viewing 1-25 of 52 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).