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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BFSP2

beaded filament structural protein 2, phakinin

More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex.

Gene Name: beaded filament structural protein 2, phakinin
Synonyms: BFSP2, 49 kDa cytoskeletal protein, Beaded filament protein CP49, CP47, CP49, LIFL-L, Phakinin, PHAKOSIN
Target Sequences: NM_003571 NP_003562.1 Q13515

Viewing 1-25 of 30 items
BFSP2 Antibody - Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human eye tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
Rabbit Polyclonal (IgG) to Human BFSP2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
Select
Rabbit Polyclonal (IgG) to Human BFSP2
Human
ELISA
Biotin Conjugated
50µg   $285.00; 100µg   $385.00
BFSP2 Antibody - IHC of paraffin-embedded Human Ovary tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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Mouse Monoclonal [clone OTI4E7] (IgG1) to Human BFSP2
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Select
Mouse Monoclonal [clone OTI4E7] (IgG1) to Human BFSP2
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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Mouse Monoclonal [clone OTI1D9] (IgG1) to Human BFSP2
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Select
Mouse Monoclonal [clone OTI1D9] (IgG1) to Human BFSP2
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - IHC of paraffin-embedded Human Kidney tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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Mouse Monoclonal [clone OTI2G2] (IgG1) to Human BFSP2
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Select
Mouse Monoclonal [clone OTI2G2] (IgG1) to Human BFSP2
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
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Mouse Monoclonal [clone OTI1C2] (IgG1) to Human BFSP2
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFSP2 mouse monoclonal antibody.  Dilution: 1:150
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Mouse Monoclonal [clone OTI1C2] (IgG1) to Human BFSP2
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
Select
Guinea pig Polyclonal (IgG) to Bovine BFSP2
Bovine, Human
IHC, IHC-Fr, WB
Unconjugated
100µl   $680.00
Select
Rabbit Polyclonal (IgG) to Human BFSP2
Human
ELISA, WB
Unconjugated
100µg   $405.00
Select
Guinea pig Polyclonal to Bovine BFSP2
Bovine
IHC, WB
Unconjugated
100µl   $485.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI3A2] (IgG1) to Human BFSP2
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY BFSP2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI3A2] (IgG1) to Human BFSP2
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI2B1] (IgG1) to Human BFSP2
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY BFSP2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI2B1] (IgG1) to Human BFSP2
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI4D3] (IgG1) to Human BFSP2
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY BFSP2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI4D3] (IgG1) to Human BFSP2
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI2B7] (IgG1) to Human BFSP2
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY BFSP2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI2B7] (IgG1) to Human BFSP2
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI1A11] (IgG1) to Human BFSP2
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
BFSP2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY BFSP2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP2.
Select
Mouse Monoclonal [clone OTI1A11] (IgG1) to Human BFSP2
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
BFSP2 Antibody - BFSP2 antibody Western Blot of 293T.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human BFSP2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
BFSP2 Antibody - Western blot analysis of extracts of mouse eye, using BFSP2 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
Select
Rabbit Polyclonal (IgG) to Human BFSP2
Mouse, Human
WB
Unconjugated
50µl   $235.00; 100µl   $295.00; 200µl   $385.00

Viewing 1-25 of 30 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).