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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATP6V1F

ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F

ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.

Gene Name: ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
Family/Subfamily: Transporter , ATPase - V type
Synonyms: ATP6V1F, ATPase, vacuolar, 14 kD, V-ATPase subunit F, Vacuolar proton pump F subunit, Vma7, V-ATPase F subunit, ATP6S14, Vacuolar proton pump subunit F, V-ATPase 14 kDa subunit, V-type proton ATPase subunit F, VATF
Target Sequences: NM_004231 NP_004222.2 Q16864

Filtered By: None
Target
ATP6V1F (18)
Reactivity
Human (17)
Mouse (1)
Rat (2)
Dog (2)
Monkey (2)
Application
IHC (3)
IHC-P (2)
WB (16)
Flo (4)
ELISA (14)
IF (2)
Host
rabbit (15)
mouse (3)
Isotype
IgG (12)
IgG1 (1)
IgG2a (2)
Clonality
monoclonal mc (3)
polyclonal pc (15)
Clone
1B8 (1)
5F10 (1)
6C11 (1)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (9)
Epitope
aa82-111 (8)
Publications
No (18)
Showing 1-18 of 18 results

ATP6V1F Antibody (clone 5F10) IHC-plus™ LS-B11773
Mouse Monoclonal [clone 5F10] (IgG2a) to Human ATP6V1F
Dog, Rat, Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.83mg/ml  $425.00
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

ATP6V1F Antibody (clone 1B8) LS-C337230
Mouse Monoclonal [clone 1B8] (IgG2a) to Human ATP6V1F
Human
Flo IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $335.00
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Human Kidney tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Human Kidney tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

ATP6V1F Antibody LS-C659719
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA Flo IHC WB
Unconjugated
100µg  $435.00

ATP6V1F Antibody (clone 6C11) LS-C172626
Mouse Monoclonal [clone 6C11] (IgG1) to Human ATP6V1F
Dog, Rat, Human, Monkey
Flo WB
Unconjugated
100µl 0.44mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody.

ATP6V1F Antibody LS-C285568
Rabbit Polyclonal (IgG) to Human ATP6V1F
Mouse
ELISA WB
Unconjugated
50µg  $285.00; 100µg  $385.00

ATP6V1F Antibody (HRP) LS-C316906
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
HRP Conjugated
50µg  $285.00; 100µg  $385.00

ATP6V1F Antibody (Biotin) LS-C316903
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

ATP6V1F Antibody (FITC) LS-C316905
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
FITC Conjugated
50µg  $285.00; 100µg  $385.00

ATP6V1F Antibody (aa82-111) LS-C319709
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
Unconjugated
200µl  $530.00

ATP6V1F Antibody (aa82-111, Biotin) LS-C252056
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

ATP6V1F Antibody (aa82-111, FITC) LS-C252057
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
FITC Conjugated
200µl  $680.00

ATP6V1F Antibody (aa82-111, APC) LS-C252054
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
APC Conjugated
200µl  $680.00

ATP6V1F Antibody (aa82-111, AP) LS-C252924
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
AP Conjugated
200µl  $680.00

ATP6V1F Antibody (aa82-111, PE) LS-C252059
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
PE Conjugated
200µl  $680.00

ATP6V1F Antibody (aa82-111, Azide-free, HRP) LS-C252058
Rabbit Polyclonal (IgG) to Human ATP6V1F
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

ATP6V1F Antibody LS-C483634
Rabbit Polyclonal to Human ATP6V1F
Human
ELISA
Unconjugated
100µl  $255.00; 200µl  $305.00; 400µl  $355.00

ATP6V1F Antibody LS-C483633
Rabbit Polyclonal to Human ATP6V1F
Human
ELISA
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

ATP6V1F Antibody (aa82-111) LS-C159141
Rabbit Polyclonal to Human ATP6V1F
Human
WB
Unconjugated
400µl  $305.00
ATP6V1F Antibody western blot of Jurkat cell line lysates (35 ug/lane). The ATP6V1F antibody detected the ATP6V1F protein (arrow).
ATP6V1F Antibody western blot of Jurkat cell line lysates (35 ug/lane). The ATP6V1F antibody detected the ATP6V1F protein (arrow).
Showing 1-18 of 18 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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