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ASAH1 / Acid Ceramidase

N-acylsphingosine amidohydrolase (acid ceramidase) 1

ASAH1 / Acid Ceramidase is a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.

Gene Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
Synonyms: ASAH1, ACDase, Acid CDase, Acid ceramidase, ASAH, Acylsphingosine deacylase, PHP32, SMAPME, PHP, Putative 32 kDa heart protein
Target Sequences: NM_004315 NP_004306.3 Q13510

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Products
Proteins (12)
Over-Expression Lysate (4)
Recombinant (8)
ASAH1 / Acid Ceramidase (12)
No (12)
10His, N-terminus + Myc, C-terminus (1)
10His-GST, N-terminus + Myc, C-terminus (1)
10His-SUMO, N-terminus + Myc, C-terminus (1)
6His-SUMO, N-terminus (1)
GST (1)
GST, N-terminus (1)
His (1)
Myc-DDK (Flag) (5)
Chimpanzee (1)
Human (7)
Mouse (2)
293T Cells (2)
E. coli (5)
HEK 293 Cells (3)
Wheat Germ Extract (2)
Purified (5)
ASAH1 / Acid Ceramidase Protein - Recombinant N-Acylsphingosine Amidohydrolase 1 By SDS-PAGE
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E. coli
His
32.7 kDa
10 µg/$276; 50 µg/$412; 100 µg/$657; 200 µg/$822; 1 mg/$1,905; 500 µg/$1,455; 5 mg/$2,871; 2 mg/$2,090
ASAH1 / Acid Ceramidase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
ASAH1 / Acid Ceramidase Protein - 12.5% SDS-PAGE of human ASAH1 stained with Coomassie Blue
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Wheat Germ Extract
GST
71 kDa
2 µg/$439
ASAH1 / Acid Ceramidase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-SUMO, N-terminus + Myc, C-terminus
100 µg/$1,128; 20 µg/$721
ASAH1 / Acid Ceramidase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
43.9 kDa
100 µg/$494
ASAH1 / Acid Ceramidase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His, N-terminus + Myc, C-terminus
47.7 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
ASAH1 / Acid Ceramidase Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
ASAH1 / Acid Ceramidase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-GST, N-terminus + Myc, C-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
ASAH1 / Acid Ceramidase Protein - Purified recombinant protein ASAH1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
46.3 kDa
20 µg/$1,107
ASAH1 / Acid Ceramidase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
43.9 kDa
20 µg/$150
ASAH1 / Acid Ceramidase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
46.3 kDa
20 µg/$150
ASAH1 / Acid Ceramidase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
46.3 kDa
100 µg/$494
Viewing 1-12 of 12 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).