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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Apg7 / ATG7

autophagy related 7

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

Gene Name: autophagy related 7
Synonyms: ATG7, Apg7, APG7L, APG7-LIKE, Autophagy related 7, Autophagy-related protein 7, HAGP7, GSA7
Target Sequences: NM_006395 NP_006386.1 O95352

Publications (2)

1
Temporal orchestration of circadian autophagy rhythm by C/EBP. Ma D, Panda S, Lin JD. The EMBO journal. 2011 30:4642-51. [PubMed:21897364] [PMC:PMC3243590] Related Antibodies: LS-C156616.
2
Autophagy mediates HIF2 alpha degradation and suppresses renal tumorigenesis. Yao J, Tripathi DN, Ding Z, Xu Y, Sun M, Zhang J, Bai S, German P, Hoang A, Zhou L, Jonasch D, Zhang X, Conti CJ, Efstathiou E, Tannir NM, Eissa NT, Mills GB, Walker CL, Jonasch E. Oncogene. Jul; [Full Text Article] [PubMed:24998849] [PMC:PMC4286517] Related Antibodies: LS-B330.

Filtered by: None
Target
Apg7 / ATG7 (122)
Reactivity
Human (113)
Mouse (75)
Rat (54)
Bovine (2)
Chicken (1)
Monkey (1)
Sheep (1)
Application
IHC (77)
IHC-Fr (1)
IHC-P (15)
WB (102)
Flo (9)
ELISA (33)
ICC (4)
IF (24)
IP (1)
Host
rabbit (109)
mouse (12)
sheep (1)
Isotype
IgG (52)
IgG2b (8)
IgG2b,k (1)
Clonality
monoclonal mc (11)
polyclonal pc (111)
Clone
1A1 (1)
29CT10.3.12 (7)
7D3E5 (1)
Format
AP Conjugated (7)
APC (1)
APC Conjugated (7)
Atto 390 Conjugated (2)
Atto 488 Conjugated (2)
Atto 565 Conjugated (2)
Atto 594 Conjugated (4)
Atto 633 Conjugated (2)
Atto 655 Conjugated (2)
Atto 680 Conjugated (2)
Atto 700 Conjugated (2)
Azide-free (3)
Biotin Conjugated (10)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (10)
HRP Conjugated (10)
PE (2)
PE Conjugated (6)
PerCP Conjugated (2)
RPE Conjugated (2)
SA Conjugated (2)
Unconjugated (46)
Epitope
aa540-569 (9)
aa477-698 (8)
Ser95 (7)
aa489-538 (4)
C-Terminus (3)
N-Terminus (2)
aa22-51 (1)
aa284-313 (1)
aa347-581 (1)
aa38-50 (1)
aa494-523 (1)
aa550-600 (1)
aa558-569 (1)
aa652-669 (1)
aa673-703 (1)
Publications
No (120)
Yes (2)
Showing 1-25 of 122 results

IHC-plus™ Apg7 / ATG7 Antibody (aa558-569) LS-B330
Publications (1)
Publications (1)
Rabbit Polyclonal to Human Apg7 / ATG7
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $485.00
Anti-ATG7 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Apg7 / ATG7 Antibody LS-B2195
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Mouse, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.

Apg7 / ATG7 Antibody (aa284-313) LS-C156616
Publications (1)
Publications (1)
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The ATG7 Antibody antibody is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L (arrow) was detected using the purified antibody.

Apg7 / ATG7 Antibody LS-C93848
Sheep Polyclonal (IgG) to Human Apg7 / ATG7
Mouse, Rat, Human
IHC, WB
Unconjugated
500µg  $325.00

IHC-plus™ Apg7 / ATG7 Antibody LS-B2194
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Mouse, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $545.00
Anti-ATG7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 ug/ml.

Apg7 / ATG7 Antibody (aa550-600) LS-C148584
Rabbit Polyclonal to Human Apg7 / ATG7
Human
IHC, IHC-P, WB
Unconjugated
100µg 0.5mg/ml  $335.00
IHC ofAPG7L in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and Polyclonal Antibody to APG7L (bottom) at 5 ug/ml.
Western blot of APG7L in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using Polyclonal Antibody to APG7L at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Apg7 / ATG7 Antibody (aa494-523) LS-C156613
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548).

IHC-plus™ Apg7 / ATG7 Antibody (C-Terminus) LS-B2216
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Mouse, Human
ICC, IHC, IHC-P, WB
Unconjugated
50µg  $515.00
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Apg7 / ATG7 Antibody (N-Terminus) LS-B2217
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Mouse, Human
ELISA, ICC, IHC, IHC-P, WB
Unconjugated
50µg  $515.00
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Apg7 / ATG7 Antibody (aa540-569) LS-C156615
Rabbit Polyclonal to Human Apg7 / ATG7
Human
IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence of U251 cells, using ATG7 Antibody. U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Apg7 / ATG7 Antibody (aa540-569) LS-C156407
Rabbit Polyclonal to Human Apg7 / ATG7
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
APG7L Antibody(D555) immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
hAPG7L-D555 (PEI 1:100)b western blot of HeLa cell line lysates (35 ug/lane). The APG7L antibody detected the APG7L protein (arrow).

Apg7 / ATG7 Antibody (aa540-569) LS-C319529
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
Unconjugated
200µl  $590.00

Apg7 / ATG7 Antibody (aa540-569, Biotin) LS-C251510
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
Biotin Conjugated
200µl  $740.00

Apg7 / ATG7 Antibody (aa540-569, FITC) LS-C251511
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
FITC Conjugated
200µl  $740.00

Apg7 / ATG7 Antibody (aa540-569, APC) LS-C251508
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
APC Conjugated
200µl  $740.00

Apg7 / ATG7 Antibody (aa540-569, AP) LS-C251509
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
AP Conjugated
200µl  $740.00

Apg7 / ATG7 Antibody (aa540-569, Azide-free, HRP) LS-C251512
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

Apg7 / ATG7 Antibody (aa540-569, PE) LS-C251514
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human
ELISA, Flo, IHC, WB
PE Conjugated
200µl  $740.00

Apg7 / ATG7 Antibody LS-C775095
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µg 1mg/ml  $365.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Apg7 / ATG7 Antibody (HRP) LS-C775093
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
HRP Conjugated
100µg 1mg/ml  $395.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Apg7 / ATG7 Antibody (Biotin) LS-C775094
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
Biotin Conjugated
100µg 1mg/ml  $395.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Apg7 / ATG7 Antibody (FITC) LS-C775097
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
FITC Conjugated
100µg 1mg/ml  $395.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Apg7 / ATG7 Antibody (AP) LS-C775091
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
AP Conjugated
100µg 1mg/ml  $395.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Apg7 / ATG7 Antibody (APC) LS-C775092
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
APC Conjugated
100µg 1mg/ml  $395.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Apg7 / ATG7 Antibody (Atto 594) LS-C775096
Rabbit Polyclonal to Human Apg7 / ATG7
Mouse, Rat, Human
IF, IHC, WB
Atto 594 Conjugated
100µg 1mg/ml  $395.00
Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.
Showing 1-25 of 122 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).