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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AP2A2

adaptor-related protein complex 2, alpha 2 subunit

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

Gene Name: adaptor-related protein complex 2, alpha 2 subunit
Synonyms: AP2A2, Adaptin, alpha B, ADTAB, Alpha-adaptin C, AP-2 complex subunit alpha-2, CLAPA2, DKFZP564D1864, HIP-9, HIP9, Huntingtin yeast partner J, HYPJ, KIAA0899, Alpha2-adaptin
Target Sequences: NM_012305 NP_036437.1 O94973

Filtered by: None
Target
AP2A2 (21)
Reactivity
Human (21)
Mouse (10)
Rat (7)
Dog (4)
Guinea pig (4)
Application
IHC (8)
IHC-P (4)
WB (21)
Flo (1)
ELISA (10)
Host
rabbit (21)
Isotype
IgG (14)
Clonality
polyclonal pc (21)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (12)
Epitope
aa610-637 (8)
aa612-661 (4)
Publications
No (21)
Showing 1-21 of 21 results

AP2A2 Antibody LS-C482433
Rabbit Polyclonal to Human AP2A2
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunohistochemical analysis of AP2-alpha-2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of AP2-alpha-2 expression in SKOV3 (A); mouse brain (B) whole cell lysates.

AP2A2 Antibody (aa610-637) LS-C156387
Rabbit Polyclonal to Human AP2A2
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human hepatocarcinoma with AP2A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of AP2A2 Antibody in HepG2 cell line lysates (35 ug/lane). AP2A2 (arrow) was detected using the purified antibody.

AP2A2 Antibody LS-C675383
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human heart tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human testis at dilution 1:100

AP2A2 Antibody LS-C659273
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, IHC, WB
Unconjugated
100µg  $435.00

AP2A2 Antibody LS-C806630
Rabbit Polyclonal (IgG) to Human AP2A2
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

AP2A2 Antibody LS-C341605
Rabbit Polyclonal to Human AP2A2
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl 1mg/ml  $385.00

AP2A2 Antibody LS-C334033
Rabbit Polyclonal (IgG) to Human AP2A2
Mouse, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human liver cancer tissue.
Western blot analysis of extracts of various cell lines.

AP2A2 Antibody LS-C748332
Rabbit Polyclonal (IgG) to Human AP2A2
Mouse, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded human liver cancer using AP2A2 antibody at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using AP2A2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

AP2A2 Antibody (aa610-637) LS-C326853
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
Unconjugated
200µl  $590.00

AP2A2 Antibody (aa610-637, Biotin) LS-C275158
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

AP2A2 Antibody (aa610-637, FITC) LS-C275160
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

AP2A2 Antibody (aa610-637, AP) LS-C275155
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
AP Conjugated
200µl  $740.00

AP2A2 Antibody (aa610-637, Azide-free, HRP) LS-C275161
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

AP2A2 Antibody (aa610-637, APC) LS-C275159
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
APC Conjugated
200µl  $740.00

AP2A2 Antibody (aa610-637, PE) LS-C275162
Rabbit Polyclonal (IgG) to Human AP2A2
Human
ELISA, WB
PE Conjugated
200µl  $740.00

AP2A2 Antibody LS-C746285
Rabbit Polyclonal (IgG) to Human AP2A2
Human
IHC-P, WB
Unconjugated
50µl  $345.00

AP2A2 Antibody LS-C764560
Rabbit Polyclonal (IgG) to Human AP2A2
Mouse, Human
WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

AP2A2 Antibody (aa612-661) LS-C135682
Rabbit Polyclonal to Human AP2A2
Mouse, Dog, Guinea pig, Rat, Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
AP2A2 antibody Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AP2A2 Antibody (aa612-661, HRP) LS-C466974
Rabbit Polyclonal to Human AP2A2
Mouse, Dog, Guinea pig, Rat, Human
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
AP2A2 antibody Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AP2A2 Antibody (aa612-661, Biotin) LS-C466972
Rabbit Polyclonal to Human AP2A2
Mouse, Dog, Guinea pig, Rat, Human
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
AP2A2 antibody Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AP2A2 Antibody (aa612-661, FITC) LS-C466973
Rabbit Polyclonal to Human AP2A2
Mouse, Dog, Guinea pig, Rat, Human
WB
FITC Conjugated
100µl 1mg/ml  $385.00
AP2A2 antibody Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-21 of 21 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).