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ALDOB

aldolase B, fructose-bisphosphate

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Gene Name: aldolase B, fructose-bisphosphate
Synonyms: ALDOB, ALDB, ALDO2, Liver-type aldolase, Aldolase 2
Target Sequences: NM_000035 NP_000026.2 P05062

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Products
Proteins (11)
Over-Expression Lysate (2)
Recombinant (9)
ALDOB (11)
No (11)
GST (2)
GST, N-terminus (1)
His (2)
His, N-Terminal (1)
His-SUMO (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (8)
Rabbit (2)
293T Cells (1)
E. coli (5)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Yeast (1)
Purified (2)
ALDOB Protein
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E. coli
His, N-Terminal
39.3 kDa
50 µg/$419
ALDOB Protein
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E. coli
His-SUMO
55.45 kD
1 mg/$1,679; 100 µg/$598; 20 µg/$405
ALDOB Protein
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Yeast
His
41.47 kD
1 mg/$1,973; 100 µg/$679; 20 µg/$429
ALDOB Protein - Recombinant Aldolase B, Fructose Bisphosphate By SDS-PAGE
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E. coli
His-T7
40.6 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
ALDOB Protein - 12.5% SDS-PAGE of human ALDOB stained with Coomassie Blue
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Wheat Germ Extract
GST
60.5 kDa
10 µg/$479; 25 µg/$670
ALDOB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
39.3 kDa
20 µg/$150
ALDOB Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
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E. coli
GST
66.5 kDa
100 µg/$502
ALDOB Protein - Purified recombinant protein ALDOB was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
39.3 kDa
20 µg/$1,107
ALDOB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
39.3 kDa
100 µg/$494
ALDOB Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).