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AANAT

aralkylamine N-acetyltransferase

AANAT belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: aralkylamine N-acetyltransferase
Synonyms: AANAT, AA-NAT, DSPS, Serotonin N-acetyltransferase, SNAT, Serotonin acetylase
Target Sequences: NM_001088 NP_001079.1 Q16613

Publications (5)

1
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation. Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC. The Journal of biological chemistry. 2001 276:24097-107. [PubMed:11313340]
2
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase. Iuvone PM, Brown AD, Haque R, Weller J, Zawilska JB, Chaurasia SS, Ma M, Klein DC. Investigative ophthalmology & visual science. 2002 43:564-72. [PubMed:11818405]
3
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin. Klein DC, Ganguly S, Coon S, Weller JL, Obsil T, Hickman A, Dyda F. Biochemical Society transactions. 2002 30:365-73. [PubMed:12196096]
4
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87). Coon SL, Del Olmo E, Young WS, Klein DC. The Journal of clinical endocrinology and metabolism. 2002 87:4699-706. [PubMed:12364461]
5
Arylalkylamine N-acetyltransferase: "the Timezyme". Klein DC. The Journal of biological chemistry. 2007 282:4233-7. [PubMed:17164235]
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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
AANAT (6)
No (6)
10His-GST, N-terminus (1)
GST, N-terminus (1)
His-T7 (2)
Myc-DDK (Flag) (2)
Human (3)
Mouse (1)
Rat (1)
293T Cells (1)
E. coli (3)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (1)
AANAT Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-GST, N-terminus
51.8 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
AANAT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
23.2 kDa
100 µg/$494
AANAT Protein - Recombinant Arylalkylamine-N-Acetyltransferase By SDS-PAGE
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E. coli
His-T7
28.4 kDa
10 µg/$279; 50 µg/$432; 100 µg/$690; 200 µg/$862; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,441
AANAT Protein - Recombinant Arylalkylamine-N-Acetyltransferase By SDS-PAGE
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E. coli
His-T7
28.2 kDa
10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,601
AANAT Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
AANAT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
23.2 kDa
20 µg/$150
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).