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Immunohistochemistry Services

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AANAT

aralkylamine N-acetyltransferase

AANAT belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: aralkylamine N-acetyltransferase
Synonyms: AANAT, AA-NAT, DSPS, Serotonin N-acetyltransferase, SNAT, Serotonin acetylase
Target Sequences: NM_001088 NP_001079.1 Q16613

Publications (5)

1
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation. Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC. The Journal of biological chemistry. 2001 276:24097-107. [PubMed:11313340]
2
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase. Iuvone PM, Brown AD, Haque R, Weller J, Zawilska JB, Chaurasia SS, Ma M, Klein DC. Investigative ophthalmology & visual science. 2002 43:564-72. [PubMed:11818405]
3
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin. Klein DC, Ganguly S, Coon S, Weller JL, Obsil T, Hickman A, Dyda F. Biochemical Society transactions. 2002 30:365-73. [PubMed:12196096]
4
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87). Coon SL, Del Olmo E, Young WS, Klein DC. The Journal of clinical endocrinology and metabolism. 2002 87:4699-706. [PubMed:12364461]
5
Arylalkylamine N-acetyltransferase: "the Timezyme". Klein DC. The Journal of biological chemistry. 2007 282:4233-7. [PubMed:17164235]
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Filtered by: None
Target
AANAT (39)
Reactivity
Human (28)
Mouse (6)
Rat (14)
Primate (5)
Application
IHC (8)
IHC-P (1)
WB (33)
ELISA (11)
ICC (6)
IF (3)
Host
rabbit (35)
mouse (4)
Isotype
IgG (17)
IgG1 (4)
Clonality
monoclonal mc (4)
polyclonal pc (35)
Clone
OTI3B9 (2)
OTI6E1 (2)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Biotin Conjugated (5)
Carrier-free (2)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (17)
Epitope
aa22-235 (8)
aa27-54 (8)
aa1-72 (4)
aa22-236 (2)
Internal (1)
Publications
No (39)
Showing 1-25 of 39 results

AANAT Antibody LS-C2610
Rabbit Polyclonal to Human AANAT
Human, Primate
IHC, WB
Unconjugated
100µl  $335.00
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AANAT Antibody (Biotin) LS-C140829
Rabbit Polyclonal to Human AANAT
Human, Primate
ICC, IHC, WB
Biotin Conjugated
100µl  $365.00
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AANAT Antibody (DY650) LS-C140752
Rabbit Polyclonal to Human AANAT
Human, Primate
ICC, IHC, WB
DY650 Conjugated
100µl  $365.00
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AANAT Antibody (DY550) LS-C140755
Rabbit Polyclonal to Human AANAT
Human, Primate
ICC, IHC, WB
DY550 Conjugated
100µl  $365.00
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AANAT Antibody (DY488) LS-C140753
Rabbit Polyclonal to Human AANAT
Human, Primate
ICC, IHC, WB
DY488 Conjugated
100µl  $365.00
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AANAT Antibody LS-C411724
Rabbit Polyclonal to Human AANAT
Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $385.00
Immunohistochemistry (IHC) analysis of AANAT antibody in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic strong positive staining. Negative control (the right) using PBS instead of primary antibody. Secondary antibody is Goat Anti-Rabbit IgG.
Immunohistochemistry (IHC) analysis of AANAT antibody in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic strong positive staining. Negative control (the right) using PBS instead of primary antibody. Secondary antibody is Goat Anti-Rabbit IgG.

AANAT Antibody LS-C671118
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, IF, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunofluorescence staining of A549 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
Positive Western Blot detected in 293 whole cell lysate, 293T whole cell lysate. All lanes: AANAT antibody at 7.5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 29 KDa. Observed band size: 29 KDa

AANAT Antibody LS-C401416
Rabbit Polyclonal (IgG) to Human AANAT
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20µl 0.2mg/ml  $205.00; 60µl 0.2mg/ml  $235.00; 120µl 0.2mg/ml  $295.00; 200µl 0.2mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human brain using AANAT Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of paraffin-embedded Human brain using AANAT Polyclonal Antibody at dilution of 1:40.

AANAT Antibody LS-C404704
Rabbit Polyclonal (IgG) to Human AANAT
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20µl 0.7mg/ml  $205.00; 60µl 0.7mg/ml  $235.00; 120µl 0.7mg/ml  $295.00; 200µl 0.7mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human cervical cancer using AANAT Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of paraffin-embedded Human cervical cancer using AANAT Polyclonal Antibody at dilution of 1:40.

AANAT Antibody (aa22-236, FITC) LS-C421311
Rabbit Polyclonal to Mouse AANAT
Mouse
ICC, WB
FITC Conjugated
100µl 0.5mg/ml  $385.00

AANAT Antibody (aa22-236, Biotin) LS-C421310
Rabbit Polyclonal to Mouse AANAT
Mouse
ICC, WB
Biotin Conjugated
100µl 0.5mg/ml  $365.00

AANAT Antibody (aa27-54) LS-C319234
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
Unconjugated
200µl  $590.00

AANAT Antibody (aa27-54, HRP) LS-C540337
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
HRP Conjugated
200µl  $740.00

AANAT Antibody (aa27-54, Biotin) LS-C520106
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

AANAT Antibody (aa27-54, FITC) LS-C250512
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

AANAT Antibody (aa27-54, APC) LS-C250511
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
APC Conjugated
200µl  $740.00

AANAT Antibody (aa27-54, PE) LS-C250513
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
PE Conjugated
200µl  $740.00

AANAT Antibody (aa27-54, AP) LS-C250510
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
AP Conjugated
200µl  $740.00

AANAT Antibody LS-C659716
Rabbit Polyclonal (IgG) to Human AANAT
Human
ELISA, WB
Unconjugated
100µg  $405.00

AANAT Antibody LS-C355621
Rabbit Polyclonal (IgG) to Rat AANAT
Rat
IF, WB
Unconjugated
50µg  $385.00
Immunofluorescence was performed on sections of adult rat pineal. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit anti-SNAT at an antibody concentration of 5-10 µg/ml diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit diluted 1:600 in TBST. Sections were then washed in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss Z2 scanning microscope at x40 objective magnification, and show immunofluorescence labelling of SNAT localized to pinealocytes. Data courtesy of Dr. James Nagy's laboratory.
Western blot of serotonin AA-NAT in transfected 293T cell lysate.

AANAT Antibody LS-C749988
Rabbit Polyclonal (IgG) to Human AANAT
Human
IF
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00

AANAT Antibody (aa1-72, clone OTI6E1) LS-C795305
Mouse Monoclonal [clone OTI6E1] (IgG1) to Human AANAT
Human
WB
Unconjugated
100µl 1mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed

AANAT Antibody (aa1-72, clone OTI6E1, Carrier-free) LS-C792369
Mouse Monoclonal [clone OTI6E1] (IgG1) to Human AANAT
Human
WB
Unconjugated, Carrier-free
100µg  $395.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT. (1:2000)

AANAT Antibody (aa1-72, clone OTI3B9) LS-C795309
Mouse Monoclonal [clone OTI3B9] (IgG1) to Human AANAT
Human
WB
Unconjugated
100µl 1mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed

AANAT Antibody (aa1-72, clone OTI3B9, Carrier-free) LS-C792372
Mouse Monoclonal [clone OTI3B9] (IgG1) to Human AANAT
Human
WB
Unconjugated, Carrier-free
100µg  $395.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT. (1:2000)
Showing 1-25 of 39 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).