Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Various types of specimens are used in immunostaining procedures, including cell suspensions, cell lines grown on culture plates or glass slides, fresh or frozen tissues, curettings, biopsies, or whole-mounted organisms. Because cells and tissues are fragile, samples need to be prepared in ways that preserve the structural integrity of the cell during the immunostaining procedure. The following protocols are recommended to prepare fresh or frozen samples for immunostaining. Samples can be stored up to a week at -20 or -80°C in the freezer, but some cellular morphology may be degraded with time, so it is best to use them as soon as possible after sectioning. If the slides are to be shipped to another laboratory, they should be shipped on dry ice.
Cell smears are useful for the detection of cell surface proteins because the cell membrane is no disrupted, preventing immunolabeling within the cytoplasm.
Cryosections are useful for the detection of proteins throughout the cell because sectioning exposes the cytoplasm.
Cryosections should be used as quickly as possible (within a few days); frozen sections can degrade with time, even when stored in the freezer. If cryosections need to be shipped to another laboratory, they should be boxed and shipped on dry ice.
For more information about LSBio Protocols contact Technical.Support@LSBio.com