Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Sequence is: Gly - Leu - Lys - Ala - Gly - Val - Ile - Ala - Val
1. Add 0.1% acetic acid/water to give a target peptide concentration of 1-5mg/mL, and sonicate if the peptide does not go into solution.
2. To any insoluble peptides add pure acetic acid to bring the concentration of acetic acid to 10 %(v/v), and sonicate if the peptide does not go into solution.
3. To any peptides still insoluble add acetonitrile to 20 %(v/v), and sonicate if the peptide does not go into solution.
4. Lyophilize any remaining insoluble peptides to remove the water, acetic acid, and acetonitrile. To the solid, add neat dimethylformamide (DMF). dropwise until the peptide dissolves. Dilute this solution slowly with water to give approximately 10% (v/v) DMF. If the peptide precipitates at any stage during this step, stop adding water and add a little more DMF until the peptide redissolves.
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.