Modified RIPA Buffer: 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 150 mM NaCl, 1% Sodium Deoxycholate, 1 mM PMSF, 1% Triton X-100, 0.1% SDS, Protease Inhibitor Cocktail (Sigma.) + SDS Loading Buffer: 50 mM Tris-HCl, pH 6.8, 0.01% Bromophenol Blue, 12.5% Glycerol, Beta-ME, 1% SDS, 5% b-mercaptoethanol
Lyophilized from 1 x modified RIPA buffer + 1 x SDS loading buffer
Centrifuge then redisolve using 200µL distiled water and boil for 2-5 min.
Store at 4°C for up 1 year. Aliquot and store reconstituted at -80°C for up to 1 year. Avoid freeze/thaw cycles.
For research use only. Intended for use by laboratory professionals.
This protein carries the LSBio 100% Guarantee.