Functional Assay
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Functional Assay
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Functional Assay
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Functional Assay
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.