Target
CCL3 / MIP-1-Alpha
Synonyms
CCL3 | C-C motif chemokine 3 | G0S19-1 | Mip1alpha | SCYA3 | SIS-beta | Chemokine (C-C motif) ligand 3 | LD78ALPHA | MIP-1-alpha | MIP1A | PAT 464.1 | Small-inducible cytokine A3
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 69 amino acid recombinant protein has a predicted molecular mass of approximately 7.8 kD. The DTT-reduced protein migrates at approximately 12 kD and non-reduced protein migrates at approximately 14 kD by SDS-PAGE. The predicted N-terminal amino acid
Purification
Greater than 97% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Recombinant mouse CCL3 (MIP-1a) chemoattracts Baf3-hCCR5 transfectants in a dose-dependent
manner. The ED50 for this effect is 0.1– 1.0 ng/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 3.0, 20 mM Glycine, 100 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee